Lsm880airyscan clsm

Confocal microscopy was performed using Zeiss LSM710 CLSM, Zeiss LSM780 CLSM and Zeiss LSM880Airyscan CLSM instruments. The settings for the imaging of Confetti fluorescent proteins were previously described (Snippert et al., 2010 (link)). The imaging of the confocal stack was done with a Zeiss LSM780 CLSM, Plan-Apochromat 3 10x/0.45 M27 Zeiss air objective.

Confocal microscopy was performed using LSM700, LSM780 CLSM and Zeiss LSM880 Airyscan CLSM instruments. The settings for imaging Brainbow fluorescent proteins were identical to those previously described in Confetti mice^{56 (link)}. The imaging of the confocal stack was done with a Zeiss LSM780 CLSM, Plan-Apochromat 3 10x/0.45 M27 Zeiss air objective. Axolotl digit imaging was performed on a Zeiss LSM780 inverted confocal microscope using a 20x/0.8NA Plan-Apochromat objective. Fluorophores were acquired as sequential image volumes. Image stacks were manually merged and aligned post-imaging using Fiji image analysis software^{59 (link)}. Axolotl digit regeneration imaging was supported by the Light Microscopy Facility, a core facility of BIOTEC/CRTD at Technische Universität Dresden.