Image lab 6.0 analysis software

SDS-PAGE and immunoblotting were performed using hippocampi from WT and TgF344-AD rats collected at either 6mo (WT: 4 female, 2 male; TgF344-AD: 2 female, 4 male) or 16mo (WT: 2 female, 4 male; TgF344-AD: 3 female, 3 male) of age. A single hippocampal lobe from each rat was bisected longitudinally, along its dorsal-ventral axis. Protein for Western blotting and amino acids for HPLC were extracted from the same half of hippocampus. Nitrocellulose membranes were incubated overnight at 4°C with primary antibody (see Supplemental Table 2). Membranes were then incubated with species-appropriate horseradish peroxidase-conjugated secondary antibodies (1:5,000). Chemiluminescent values of the protein of interest were divided by its corresponding β-actin chemiluminescent values (Chemidoc XRS+ Imager equipped with ImageLab 6.0 analysis software; Bio-Rad). The ratio of each experimental sample was divided by the average of all the control sample values in each gel and multiplied by 100. The average of the normalized control values from each gel was 100% ± SEM. The normalized values were then averaged and used for statistical analysis.