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Axio zoom widefield microscope

Manufactured by Zeiss
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The Axio Zoom widefield microscope is a high-performance optical instrument designed for a wide range of applications. It features a zoom function that allows for continuous magnification adjustment, enabling users to observe samples at different magnification levels. The microscope is equipped with advanced optics and illumination systems to provide clear, high-quality images.

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Lab products found in correlation

Cm3050s cryostat, by Leica (4 mentions) Rabbit anti gfp, by Abcam (4 mentions) Lsm 700 confocal microscope, by Zeiss (4 mentions) Anti mouse igg conjugated to alexa fluor 647, by Thermo Fisher Scientific (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (3 mentions) Fluoromount mounting medium, by Merck Group (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti rabbit immunoglobulin g igg conjugated to alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Camkii α 6g9 mouse monoclonal antibody mab, by Cell Signaling Technology (1 mentions)

7 protocols using axio zoom widefield microscope

1

Perfusion, Fixation, and Sectioning of Rat Brains

Cited 2 times
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Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold PBS, and followed by ice-cold 4% PFA (paraformaldehyde) in PBS. Brains were fixated in PFA overnight and transferred to 30% sucrose in PBS to equilibrate for three days as previously described [6 (link), 7 (link), 9 (link)]. Following this, 20 µm coronal sections were collected by using Leica CM3050s cryostat (Leica Biosystems, Germany) and washed with PBS for 10 min. Sections were washed in PBS and coverslipped with Vectashield mounting medium. Sections were made after viral transfer for opsin verification, and these were stained with anti-rabbit GFP (1:500, #AB290, Abcam, USA). Secondary antibody was anti-rabbit immunoglobulin G (Ig G) conjugated to Alexa Fluor 488 (1:500, Life Technologies, USA). Images were acquired with a Zeiss LSM 700 Confocal Microscope (Carl Zeiss, Thornwood, NY, USA). Images containing cannulas were stained with cresyl violet and examined at 10 × magnification with an Axio Zoom widefield microscope (Carl Zeiss, Thornwood, NY, USA).
Zeng F., Zhang Q., Liu Y., Sun G., Li A., Talay R.S, & Wang J. (2021). AMPAkines potentiate the corticostriatal pathway to reduce acute and chronic pain. Molecular Brain, 14, 45.
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2

Brain Tissue Preparation and Immunohistochemistry

Cited 1 time
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Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold phosphate-buffered saline (PBS) and paraformaldehyde (PFA). After extraction, brains were fixed in 4% PFA overnight and then transferred to 30% sucrose in PBS to be cryoprotected for 3 days66 (link). Next, 20 μm coronal sections were collected using Leica CM3050S cryostat (Leica Biosystems), washed in PBS, and coverslipped with Vectashield mounting medium. Sections from brains containing electrodes were stained with cresyl violet and imaged at 10x magnification with an Axio Zoom widefield microscope (Carl Zeiss). Sections also were made after viral transfer for opsin verification, and these sections were stained with anti-rabbit GFP (1:500, #AB290, Abcam), CaMKII-a (6G9) mouse monoclonal antibody (mAb) (1:200, #50049, Cell Signaling Technology) antibodies, and coverslipped with DAPI (1:200, Vector Laboratories). Secondary antibodies were anti-rabbit immunoglobulin G (IgG) conjugated to Alexa Fluor 488, and anti-mouse IgG conjugated to Alexa Fluor 647 (1:200, Life Technologies). Images were acquired with a Zeiss LSM 700 confocal microscope (Carl Zeiss).
Zhang Q., Hu S., Talay R., Xiao Z., Rosenberg D., Liu Y., Sun G., Li A., Caravan B., Singh A., Gould J.D., Chen Z.S, & Wang J. (2021). A prototype closed-loop brain–machine interface for the study and treatment of pain. Nature biomedical engineering, 7(4), 533-545.
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3

Perfusion-Based Brain Tissue Preparation

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Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold PBS and paraformaldehyde (PFA). After extraction, brains were fixed in PFA overnight and then cryoprotected in 30% sucrose in PBS for 48 h or until sinking (Lee et al., 2015 (link)). 20 μm coronal sections were washed in PBS and coverslipped with Fluoromount mounting medium (Sigma-Aldrich). Images containing cannula were stained with cresyl violet. Images were acquired with an Axio Zoom widefield microscope (Carl Zeiss).
Li A., Liu Y., Zhang Q., Friesner I., Jee H.J., Chen Z.S, & Wang J. (2021). Disrupted population coding in the prefrontal cortex underlies pain aversion. Cell reports, 37(6), 109978.
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4

Perfusion-Based Brain Tissue Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold PBS and paraformaldehyde (PFA). After extraction, brains were fixed in PFA overnight and then cryoprotected in 30% sucrose in PBS for 48 h or until sinking (Lee et al., 2015 (link)). 20 μm coronal sections were washed in PBS and coverslipped with Fluoromount mounting medium (Sigma-Aldrich). Images containing cannula were stained with cresyl violet. Images were acquired with an Axio Zoom widefield microscope (Carl Zeiss).
Li A., Liu Y., Zhang Q., Friesner I., Jee H.J., Chen Z.S, & Wang J. (2021). Disrupted population coding in the prefrontal cortex underlies pain aversion. Cell reports, 37(6), 109978.
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5

Brain Tissue Preparation and Immunohistochemistry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold phosphate-buffered saline (PBS) and paraformaldehyde (PFA). After extraction, brains were fixed in 4% PFA overnight and then transferred to 30% sucrose in PBS to be cryoprotected for 3 days66 (link). Next, 20 μm coronal sections were collected using Leica CM3050S cryostat (Leica Biosystems), washed in PBS, and coverslipped with Vectashield mounting medium. Sections from brains containing electrodes were stained with cresyl violet and imaged at 10x magnification with an Axio Zoom widefield microscope (Carl Zeiss). Sections also were made after viral transfer for opsin verification, and these sections were stained with anti-rabbit GFP (1:500, #AB290, Abcam), CaMKII-a (6G9) mouse monoclonal antibody (mAb) (1:200, #50049, Cell Signaling Technology) antibodies, and coverslipped with DAPI (1:200, Vector Laboratories). Secondary antibodies were anti-rabbit immunoglobulin G (IgG) conjugated to Alexa Fluor 488, and anti-mouse IgG conjugated to Alexa Fluor 647 (1:200, Life Technologies). Images were acquired with a Zeiss LSM 700 confocal microscope (Carl Zeiss).
Zhang Q., Hu S., Talay R., Xiao Z., Rosenberg D., Liu Y., Sun G., Li A., Caravan B., Singh A., Gould J.D., Chen Z.S, & Wang J. (2021). A prototype closed-loop brain–machine interface for the study and treatment of pain. Nature biomedical engineering, 7(4), 533-545.
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6

Perfusion, Fixation, and Imaging of Rodent Brains

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Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold PBS and paraformaldehyde (PFA). After extraction, brains were fixed in PFA overnight and then cryoprotected in 30% sucrose in PBS for 48 hours or until sinking [60 (link)]. 20 μm coronal sections were washed in PBS and coverslipped with Vectashield mounting medium. Sections from brains containing tetrodes were stained with cresyl violet and imaged at 10x magnification with an Axio Zoom widefield microscope (Carl Zeiss). Sections also were made after viral transfer for opsin verification, and these sections were stained with anti-rabbit GFP (1:500, #AB290, Abcam), CaMKII-α (6G9) mouse monoclonal antibody (mAb) (1:200, #50049, Cell Signaling Technology) antibodies, and cover-slipped with DAPI (1:200, Vector Laboratories). Secondary antibodies were anti-rabbit immunoglobulin G (IgG) conjugated to Alexa Fluor 488, and anti-mouse IgG conjugated to Alexa Fluor 647 (1:200, Life Technologies). Sections with RetroBeads solution (Lumafluor) injection were cover-slipped with DAPI (1:200, Vector Laboratories). Images were acquired with a Zeiss LSM 700 confocal microscope (Carl Zeiss).
Singh A., Patel D., Li A., Hu L., Zhang Q., Liu Y., Guo X., Robinson E., Martinez E., Doan L., Rudy B., Chen Z.S, & Wang J. (2020). Mapping Cortical Integration of Sensory and Affective Pain Pathways. Current biology : CB, 30(9), 1703-1715.e5.
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7

Perfusion and Cryosectioning for Brain Imaging

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Rats were deeply anesthetized with isoflurane and transcardially perfused with ice-cold PBS. Brains were fixated in paraformaldehyde (PFA) overnight and then transferred to 30% sucrose in PBS to equilibrate for three days as described (Lee et al., 2015 (link)). After, 20 μm coronal sections were collected using Leica CM3050s cryostat (Leica Biosystems). Images containing electrodes and cannulas were stained with cresyl violet and virus expression was viewed using an Axio Zoom widefield microscope (Carl Zeiss).
Talay R.S., Liu Y., Michael M., Li A., Friesner I.D., Zeng F., Sun G., Chen Z.S., Zhang Q, & Wang J. (2021). Pharmacological restoration of anti-nociceptive functions in the prefrontal cortex relieves chronic pain. Progress in neurobiology, 201, 102001.
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