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Electrochemiluminescence detection technology

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Electrochemiluminescence detection technology is an analytical technique used to measure the light emitted from chemical reactions driven by an applied electrical potential. It provides a sensitive and specific method for detecting and quantifying various analytes in a sample.

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Quickplex sq 120, by Mesoscale (2 mentions)

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Electrochemiluminescence technology (5 citations) MULTI-ARRAY electrochemiluminescence detection technology (2 citations)

2 protocols using electrochemiluminescence detection technology

1

Quantifying Poly(GP) Levels in Lysates

Cited 1 time
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Poly(GP) levels in lysates were measured in a blinded fashion using a previously described sandwich immunoassay that utilizes Meso Scale Discovery electrochemiluminescence detection technology, and an affinity purified rabbit polyclonal poly(GP) antibody (Rb9259) as both capture and detection antibody 25 –28 (link). Lysates were diluted to the same concentration using Tris-buffered saline (TBS) and tested in duplicate wells. Response values corresponding to the intensity of emitted light upon electrochemical stimulation of the assay plate using the Meso Scale Discovery QUICKPLEX SQ120 were acquired. All responses were background corrected using the response from the negative control samples. In some cases when comparing across mutants or iPSC lines, poly(GP) responses were then normalized to our positive control.
Yamada S.B., Gendron T.F., Niccoli T., Genuth N.R., Grosely R., Shi Y., Glaria I., Kramer N.J., Nakayama L., Fang S., Dinger T.J., Thoeng A., Rocha G., Barna M., Puglisi J.D., Partridge L., Ichida J.K., Isaacs A.M., Petrucelli L, & Gitler A.D. (2019). RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. Nature neuroscience, 22(9), 1383-1388.
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2

Quantifying Poly(GP) Levels in Lysates

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Poly(GP) levels in lysates were measured in a blinded fashion using a previously described sandwich immunoassay that utilizes Meso Scale Discovery electrochemiluminescence detection technology, and an affinity purified rabbit polyclonal poly(GP) antibody (Rb9259) as both capture and detection antibody 25 –28 (link). Lysates were diluted to the same concentration using Tris-buffered saline (TBS) and tested in duplicate wells. Response values corresponding to the intensity of emitted light upon electrochemical stimulation of the assay plate using the Meso Scale Discovery QUICKPLEX SQ120 were acquired. All responses were background corrected using the response from the negative control samples. In some cases when comparing across mutants or iPSC lines, poly(GP) responses were then normalized to our positive control.
Yamada S.B., Gendron T.F., Niccoli T., Genuth N.R., Grosely R., Shi Y., Glaria I., Kramer N.J., Nakayama L., Fang S., Dinger T.J., Thoeng A., Rocha G., Barna M., Puglisi J.D., Partridge L., Ichida J.K., Isaacs A.M., Petrucelli L, & Gitler A.D. (2019). RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats. Nature neuroscience, 22(9), 1383-1388.
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