G1492

Manufactured by Solarbio

Sourced in China

G1492 is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Safranin o fast green, by Solarbio (1 mentions) G1120, by Solarbio (1 mentions) C3206, by Beyotime (1 mentions) G1452, by Solarbio (1 mentions)

5 protocols using g1492

Quantifying Osteoclast Activity via TRAP

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

TRAP staining was carried out according to the manufacturer’s instructions (G1492, Solarbio, Beijing, China). The area of the callus was defined as the region of interest. TRAP-positive cells were counted using image analysis software.22 (link)

Liu H., Tian F., Hu Y., Ping S, & Zhang L. (2023). Liraglutide in Combination with Insulin Has a Superior Therapeutic Effect to Either Alone on Fracture Healing in Diabetic Rats. Diabetes, Metabolic Syndrome and Obesity, 16, 1235-1245.

+ Open protocol

+ Expand

Osteoclast Density Evaluation in Bone Callus

Check if the same lab product or an alternative is used in the 5 most similar protocols

The same steps (see “Histological analysis” section) were taken to process the slices, and then Tartrate-resistant acid phosphatase (TRAP) staining was performed to evaluate the density of osteoclasts in the callus area following a standard protocol (G1492, Solarbio, Beijing, China). Measurement processes were performed as previously described [19 (link)].

Hu Y., Lian Q., Cao F., Hou X., Li H., Xing L., Wang M., Tian F, & Zhang L. (2023). Estrogen deficiency impedes fracture healing despite eliminating the excessive absorption of the posterior callus in a semi-fixed distal tibial fracture mouse model. BMC Musculoskeletal Disorders, 24, 803.

+ Open protocol

+ Expand

Bone metabolism evaluation protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Medicine refrigerator (BYC-310, Shandong Boke Biology), refrigerated freezer (BD/BC-415DKEM, Midea), slicer (BQ-318D, Bernard), electric blast drying oven (HGZF-101-1, Shanghai Yuejin Medical Instruments Co., Ltd.), thermostatic incubator (DHP-9054, Shandong Boke Biology), electric thermostatic incubator (DHP-9054, Shandong Boke Biology Industry Co., Ltd.); Pressure cooker (YS20ED, Suber), induction cooker (HK-22, Hanke Electric Appliance Factory, Dongfeng Town, Zhongshan City), fume hood; Microscope (CX43, OLYMPUS), microtome (2235, Leica), multifunctional enzyme marker (SuPerMax 3100, flash spectrum); First antibody: BMP-2 (AF5163, Affinity); leptin(DF8583, Affinity); Sema4d (PAB430Ra01, cloud clone); Second antibody: horseradish enzyme-labeled goat anti-rabbit IgG (H + L) (ZB-2301, Zhongshan Jinqiao, 1/100); Tartrate-resistant acid phosphatase staining solution (G1492, Solarbio); Rat tartrate-resistant acid phosphatase 5b (TRACP-5b) ELISA reagent (MM-70644R1, enzyme immunoassay); Rat bone alkaline phosphatase (BALP) kit (MM-0619R1, enzyme immunoassay); Melatonin (S20287) was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with a relative molecular weight of 232.28 and a purity of more than 99%.

Lin Z., Xiong S., Lin Y., Li Z., Xie D., Lin X., Chen X, & Lin X. (2023). Impact of leptin or melatonin on Sema4D overexpression-related bone metabolism. Journal of Orthopaedic Surgery and Research, 18, 285.

+ Open protocol

+ Expand

Histological Analysis of Bone Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hydrated bone sections were stained for hematoxylin and eosin staining (Solarbio, G1120), TRAP staining (Solarbio, G1492) and Safranin O/Fast green staining (Solarbio, G1371) according to the manufacturer’s directions, and analyzed by the Bioquant Osteo software (Bioquant Image Analysis Corp, USA).

Fu Y., Li L., Zhang X., Deng Z., Wu Y., Chen W., Liu Y., He S., Wang J., Xie Y., Tu Z., Lyu Y., Wei Y., Wang S., Cui C.P., Liu C.H, & Zhang L. (2024). Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis. Nature Communications, 15, 2974.

+ Open protocol

+ Expand

Quantifying Osteoclast Activity and Mineralization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell plates or histological sections were stained with a tartrate‐resistant acid phosphatase (TRAP) staining solution (G1492‐4 × 20 mL, Solarbio, Beijing, China) for 40 min (cell plate) or 20 min (slides) followed by hematoxylin or methyl green following the manufacturer's instructions. Positive multinucleated osteoclasts (≥3 nuclei) were observed and counted.¹³, ³³ Immunohistochemically stained slices from the proximal epiphyses and growth plates of the proximal tibiae were analyzed.¹³, ²² The osteoclast numbers and osteoclast surface areas were assessed using ImageJ (USA). Alkaline phosphatase (ALP) staining (C3206, Beyotime, Haimen, China) and staining of mineralized nodules with alizarin red (G1452, Solarbio, Beijing, China) were performed following the manufacturers' instructions. The proximal tibiae were analyzed using a 1500×1800‐μm region of interest (ROI) 150 μm from the growth plate in a grid‐like pattern along the ROI.³⁴ Five fields of each slide and five slides were randomly selected for analysis.³⁵

Yuan S., Hu H., Wang X., Yang J., Zhou R., Bai X, & Lai P. (2022). Bindarit Reduces Bone Loss in Ovariectomized Mice by Inhibiting CCL2 and CCL7 Expression via the NF‐κB Signaling Pathway. Orthopaedic Surgery, 14(6), 1203-1216.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!