Blood samples were drawn at the baseline visit and centrifuged to obtain plasma. Aliquots were stored at –80°C until the samples were shipped to Quanterix Corporation (113 Hartwell Avenue, Lexington, MA, USA, techsupport@quanterix.com ) for analysis of pNfL utilizing the Simoa NF-light kit on the Quanterix Simoa HD-1 Analyzer. Concentrations of pNfL were measured, in units of pg/ml, in duplicate from each sample, that were blinded to demographic and clinical data. All samples tested were within the assay dynamic range with an average coefficient of variation of 3.9% (range from 0 to 15% for measurement.
Simoa nf light kit
Manufactured by Quanterix
Sourced in United States
The SIMOA Nf-light® kit is a lab equipment product offered by Quanterix. It is designed to detect and quantify neurofilament light (Nf-light) protein levels in biological samples. The kit provides the necessary reagents and components to perform this analysis using the SIMOA technology.
Automatically generated - may contain errors
Lab products found in correlation
Simoa hd 1 analyzer, by Quanterix (1 mentions)
Simoa sr x analyzer, by Quanterix (1 mentions)
Filtermax f3 microplate reader, by Molecular Devices (1 mentions)
Lumipulse g1200, by Fujirebio (1 mentions)
Simoa hd 1 analyser, by Quanterix (1 mentions)
Innovance d dimer, by Siemens (1 mentions)
Cobas c701, by Roche (1 mentions)
Simoa hd x analyser, by Quanterix (1 mentions)
Matrix 2d barcode tube 1.0 ml, by Thermo Fisher Scientific (1 mentions)
Hd 1 analyzer, by Quanterix (1 mentions)
Simoa ptau181 advantage kit, by Quanterix (1 mentions)
Hiscl 5000, by Sysmex (1 mentions)
Simoa gfap discovery kit, by Quanterix (1 mentions)
Sr x immunoassay analyzer, by Quanterix (1 mentions)
14 protocols using simoa nf light kit
1
Plasma Neurofilament Light Quantification
2
Quantification of Neurofilament Light in CSF and Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NfL was measured in CSF and serum using the Simoa Nf-light kit and provided consumables in the Simoa SR-X Analyzer (Quanterix, Lexington, MA, USA).26 (link) The NfL assay was performed according to the manufacturer's instructions and protocol, as previously described.27 All samples were measured under blinded conditions at the Medical University of Vienna, Department of Neurology.
As sNfL concentrations increase with age and decrease with BMI under physiological conditions, we calculated age- and BMI-adjusted Z scores. This allows to quantify the deviation of each patient's individual sNfL value in comparison to control persons of the same age and BMI, based on a recently published reference database.19 (link) Z score >1.5 was defined as elevated (termed as ‘positive’), Z score ≤1.5 was defined as ‘negative’. A Z score >3 was considered as ‘high’, a Z score >1.5 and ≤3 was considered as ‘moderately elevated’.
As sNfL concentrations increase with age and decrease with BMI under physiological conditions, we calculated age- and BMI-adjusted Z scores. This allows to quantify the deviation of each patient's individual sNfL value in comparison to control persons of the same age and BMI, based on a recently published reference database.19 (link) Z score >1.5 was defined as elevated (termed as ‘positive’), Z score ≤1.5 was defined as ‘negative’. A Z score >3 was considered as ‘high’, a Z score >1.5 and ≤3 was considered as ‘moderately elevated’.
3
Quantification of Plasma Neurofilament Light
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of plasma NfL were determined in plasma samples from n = 123 individuals using standardized service protocols and the Simoa™ NF-light® Kit (Quanterix) at PBL Assay Science (Piscataway, NJ, USA). Intra- and inter-assay variations were 4% and 9% respectively.
4
Plasma Biomarker for Neurodegeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess neurodegeneration, plasma samples were sent to PBL Assay Science (Piscataway, NJ, USA) and analyzed using the Simoa™ NF-Light® kit (Cat #103186, Quanterix, Billerica, MA, USA).
5
Biomarker Measurements in Cerebrospinal Fluid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CSF Aβ1–42, Aβ1–40, tTau, and pTau181 were measured using LUMIPULSE G1200 chemiluminescent ELISA (Fujirebio, Malvern, PA) directly from the cryovials without tube transfer. A CSF internal control was run on each day that samples were analyzed. The coefficients of variation were as follows: Aβ1–42 3.4%, Aβ1–40 2.7%, tTau 8%, and pTau181 1.8%. CSF NfL was measured with the Simoa NF-Light Kit using the SRX platform (Quanterix, Billerica, MA). Intra-assay coefficients of variation were 6.1% and 2.3%, and interassay coefficients of variation were <10% for quality control samples with clinically relevant low and high concentrations, respectively. CSF LRG1was measured by a solid-phase sandwich ELISA, Human LRG1 Assay Kit 27769 (IBL America, Minneapolis, MN). The plate was analyzed using a FilterMax F3 microplate reader (Molecular Devices, San Jose, CA). The intra-assay coefficient of variation was <10%, but the interassay coefficient of variation was 30% for the internal native CSF quality control sample due to individual preparation of controls. Hence, results were normalized across plates.
6
Ultrasensitive SIMOA Assay for NFL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Measurement of NFL concentration was performed in duplicates in all sera of included patients and HC. NFL concentration was analyzed using SIMOA Nf-light® kit in SR-X immunoassay analyzer, Simoa TM (Quanterix Corp, Boston, MA), which runs ultrasensitive paramagnetic bead-based enzyme-linked immunosorbent assays. Briefly, frozen samples and calibrator were equilibrated to room temperature and diluted with specific sample diluent. Calibrators, samples, detector, and beads were dispensed in each well and plates were incubated at 30 °C with shaking 800 revolutions per minute (rpm) for 30 min. After washing steps, 100 μl SBG was added to each well and plates were incubated at 30 °C with shaking 800 rpm for 10 min. After washing steps, beads were resuspended twice at 1000 rpm for 1 min. A final washing step was performed and plates were dried for 10 min before being transferred to the SR-X for reading.
7
Serum Biomarkers in CIDP Diagnosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected from patients with CIDP and HC, immediately centrifuged, and stored at −80°C until being analyzed in May and February 2021. Serum levels of CLP were measured using the fCAL turbo method? on a COBAS 8000 semi-automated analyses (Bühlmann Laboratories AG, Schönenbuch, Switzerland) according to the manufacturer's protocol (37 (link), 38 (link)). The fCAL turbo method? has been validated for accurate measurement of CLP levels in serum (37 (link)). sNfl concentrations were measured using SIMOA Nf-light kit® in SR-S immunoassay analyzer, SIMOA™ (Quanterix Corp., Boston, MA, USA) according to the manufacturer's protocol (39 (link)). SIMOA Nf-light kit® is an ultrasensitive paramagnetic bead-based enzyme-linked immunosorbent assay, is at least 125 times more sensitive than conventional immunoassays, and maintains a high analytical performance (40 (link)).
8
Quantification of Neurofilament Light by Simoa
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For NfL measures, samples were thawed for 60 min at room temperature and were analysed by an investigator blinded to clinical data using the Simoa Nf-light kit in the Simoa HD-1 Analyser (Quanterix, Lexington, MA, USA), which runs ultrasensitive paramagnetic bead-based enzyme-linked immunosorbent assays [27 (link)]. For this protocol, briefly, thawed samples and calibrators were dispensed in provided 96-well plates as duplicates. Further sample processing (dilution, incubation, washing, shaking, resuspending and reading) was carried out in an automated manner as described elsewhere [28 (link)]. The sNfL assay was carried out according to the manufacturer’s instructions and protocol.
9
Quantification of Neuroaxonal Damage in COVID-19
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quantification of neurofilament light chain (NF-L) in CSF, a marker of neuroaxonal damage [42 (link)], was used as indicator of neuronal injury in COVID-19 and control subjects. CSF NFL was measured simultaneously in both COVID-19 and control samples using the Simoa™ NF-Light Kit (Quanterix Corporation, Lexington, MA, USA) on the Quanterix HD-X® platform. Acute phase reactants such as ferritin, C-reactive protein (CRP) and coagulation markers including D-dimer, fibrinogen and factor VIII, markers associated with disease severity in COVID-19 [[43] (link), [44] (link), [45] (link), [46] (link)] were also evaluated in CSF of COVID-19 and control subjects. Ferritin and hsCRP were measured on Roche Diagnostics Cobas c 701 and e 801 analyzers, respectively. Fibrinogen quantification used a clot-based assay (Siemens, Marburg Germany). D-dimer was measured by an immunoturbidimetric assay (Innovance D-Dimer, Siemens, Marburg, Germany). Factor VIII assessment used a chromogenic Assay (Chromogenix, Bedford, MA).
10
Quantification of Neurofilament Light in CSF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CSF was collected via the cisterna magna at the end of the in vivo imaging procedure and stored at − 80 °C. Upon analysis, samples were thawed and 12.5 × diluted in sample diluent provided with the kit. Neurofilament light (NfL) levels were measured using the Simoa™ NF-light® kit on a Simoa® HD-X analyser (Quanterix Corp., Billerica, MA, U.S.A.), according to the manufacturer’s instructions. During this run, CSF samples were automatically diluted another 4 times, resulting in a final dilution of 50 ×.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!