The Mayfield cranial stabilization and fixation system manufactured by Integra (Cincinnati, OH) is used to position the pediatric patient for surgical biopsy. The Stealth Vertek biopsy device, manufactured by Medtronic (Minneapolis, MN) is employed to collect specimens. Eight cores are typically acquired, as described in the Results section. Each needle core biopsy collected for cell culture and direct orthotopic implants is stored in RPMI (containing 500 mL RPMI-1640 with L-glutamine, and no phenol red (ThermoFisher cat.# 11835-030), 50 mL aliquot fetal calf serum (HyClone SH30070.03), 5 mL penicillin-streptomycin solution (ThermoFisher 15140-122), and 5 mL gentamicin (Sigma G1272)) at 4°C until ready for continued processing. Standard hematoxylin and eosin (H&E) staining is performed on primary biopsy tissue to assess tumor cell morphology. Staining for the diagnostic H3 K27M protein is performed with a 1:1200 antibody dilution (EMD Millipore cat.# ABE419) using the iView DAB detection kit (Roche cat. #760-091) on the Ventana Benchmark Ultra automated platform.
Abe419
Manufactured by Roche
The ABE419 is a laboratory equipment product manufactured by Roche. It is designed for general laboratory use. The core function of the ABE419 is to perform specific tasks required in a laboratory setting. No further details about the intended use or capabilities of the product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using abe419
1
Pediatric Brain Tumor Biopsy Protocol
2
Pediatric Brain Tumor Biopsy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Mayfield cranial stabilization and fixation system manufactured by Integra (Cincinnati, OH) is used to position the pediatric patient for surgical biopsy. The Stealth Vertek biopsy device, manufactured by Medtronic (Minneapolis, MN) is employed to collect specimens. Eight cores are typically acquired, as described in the Results section. Each needle core biopsy collected for cell culture and direct orthotopic implants is stored in RPMI (containing 500 mL RPMI-1640 with L-glutamine, and no phenol red (ThermoFisher cat.# 11835-030), 50 mL aliquot fetal calf serum (HyClone SH30070.03), 5 mL penicillin-streptomycin solution (ThermoFisher 15140-122), and 5 mL gentamicin (Sigma G1272)) at 4°C until ready for continued processing. Standard hematoxylin and eosin (H&E) staining is performed on primary biopsy tissue to assess tumor cell morphology. Staining for the diagnostic H3 K27M protein is performed with a 1:1200 antibody dilution (EMD Millipore cat.# ABE419) using the iView DAB detection kit (Roche cat. #760-091) on the Ventana Benchmark Ultra automated platform.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!