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Caspase glo 3 7 3d assay

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The Caspase-Glo® 3/7 3D Assay is a luminescent-based assay that measures the activity of caspase-3 and caspase-7 in 3D cell culture models. The assay is designed to quantify caspase-3/7 activity in a homogeneous, add-mix-measure format, providing a simple and convenient method for researchers to assess apoptosis in their 3D cell culture systems.

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Lab products found in correlation

Caspase glo 3 7 assay, by Promega (1 mentions) Fluostar optima microplate reader, by BMG Labtech (1 mentions) Caspase glo 3 7 assay system, by Promega (1 mentions) Celltiter glo assay, by Promega (1 mentions) Fluostar omega plate reader, by BMG Labtech (1 mentions)

Close spelling variants of this product

Caspase-Glo assay (87 citations) Caspase-Glo (67 citations) Caspase 3/7 assay (26 citations) Caspase-3 (7 citations) Caspase-3 Glo (2 citations) Caspase-Glo® 3/7 3D Assay kit (2 citations)

3 protocols using caspase glo 3 7 3d assay

1

Caspase-3/7 Assay for Apoptosis Detection

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Apoptosis was assessed using the Caspase-Glo® 3/7 Assay (G8090, Promega GmbH, Walldorf, Germany) and Caspase-Glo® 3/7 3D Assay (G8981, Promega GmbH, Walldorf, Germany), utilizing a luminogenic caspase-3/7 substrate which contains the tetrapeptide sequence DEVD in a reagent optimized for caspase activity, luciferase activity, and cell lysis. Cells were seeded in 96-well plates at a density of 4000 cells/well for U87 and U251 and at a density of 10,000 cells/well for GSCs. 24 h after seeding, cells were treated with the drugs. For U87 and U251, apoptosis was measured 24 h after treatment. For GSCs, apoptosis was measured 48 h after treatment. For this, 20 µL of the reagent was added to each well (Caspase-Glo® 3/7 Assay for U87 and U251, and Caspase-Glo® 3/7 3D Assay for GBM Stem-Like Cells). The contents were mixed gently on a platform shaker for 30 s, followed by a one-hour incubation at room temperature in the dark. Luminescence was recorded using a FLUOstar OPTIMA Microplate Reader (BMG LABTECH, Ortenberg, Germany). Equivalent to the viability assay, data were normalized to the control group.
Otte K., Zhao K., Braun M., Neubauer A., Raifer H., Helmprobst F., Barrera F.O., Nimsky C., Bartsch J.W, & Rusch T. (2022). Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide. Biomedicines, 10(9), 2145.
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2

Caspase 3/7 Activation in GBM Cells

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The induction of apoptosis by LTZ, TMZ and LTZ + TMZ combination as evident by caspase 3/7 activation was assessed in all four GBM lines, G76, BT142, G43 and G75. Cells were seeded at a density of 1.5 × 104 cells per well in a translucent walled, clear bottom, 96-well plate. Promega Caspase-Glo® 3/7 Assay system was employed to assess the Caspase 3/7 levels in cells treated with LTZ, TMZ, LTZ (40 nM) + TMZ (0–5 μM) and vehicle (0.1% DMSO) [31 (link)]. Cells were treated for 24 h after plating. Caspase-Glo® 3/7 3D substrate and Caspase-Glo® 3/7 3D buffer was mixed to form a 10 mL reaction mixture and 100 μL of the mixture was added to each well. The reaction was allowed to proceed at room temperature in the dark for 30 min as described in the Promega Caspase-Glo® 3/7 3D-Assay technical manual. The luminescence readings were carried out 24 h after the treatment using Synergy™ HTX Multi-Mode Microplate Reader. The experiment was repeated in presence of estradiol to assess the effect of estradiol on caspase induction in in four GBM lines used in this study. The data were normalized to control using Promega CellTiter- Glo® assay.
Karve A.S., Desai J.M., Dave N., Wise-Draper T.M., Gudelsky G.A., Phoenix T.N., DasGupta B., Sengupta S., Plas D.R, & Desai P.B. (2022). Potentiation of temozolomide activity against glioblastoma cells by aromatase inhibitor letrozole. Cancer chemotherapy and pharmacology, 90(4), 345-356.
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3

Apoptosis Quantification in Organoids

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The apoptosis levels of organoids were measured using Caspase-Glo 3/7 3D Assay (G9681, Promega) according to the manual's standard protocol for Matrigel-embedded microtissues. Mixing of the reagent with Matrigel was enhanced by pipetting the reagent ten times in each well. Caspase 3/7 levels were measured with FLUOstar Omega plate reader (BMG Labtech).
Lemmetyinen T.T., Viitala E.W., Wartiovaara L., Kaprio T., Hagström J., Haglund C., Katajisto P., Wang T.C., Domènech-Moreno E, & Ollila S. (2023). Fibroblast-derived EGF ligand neuregulin 1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth. Disease Models & Mechanisms, 16(4), dmm049692.
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