All compounds and aripiprazole were synthesized as described under General Chemistry Procedures. Dopamine hydrochloride, (−)-quinpirole, (+)-butaclamol hydrochloride, 5-hydroxytryptamine creatine sulfate, (−)-isoproterenol bitartrate, and HEPES sodium salt were purchased from Sigma-Aldrich (St. Louis, MO). HBSS (10X) was purchased by Invitrogen and fatty-acid free BSA was purchased from Akron Biotech.
Isoproterenol bitartrate
Manufactured by Merck Group
Sourced in Sao Tome and Principe
(−)-isoproterenol bitartrate is a chemical compound used as a laboratory reagent. It is the (−)-enantiomer of the sympathomimetic drug isoproterenol. The compound is typically used in scientific research and experiments, but its specific applications may vary.
Automatically generated - may contain errors
Lab products found in correlation
Quinpirole, by Merck Group (2 mentions)
Hepes sodium salt, by Merck Group (2 mentions)
Butaclamol hydrochloride, by Merck Group (2 mentions)
5 hydroxytryptamine creatine sulfate, by Merck Group (2 mentions)
Hbss 10x, by Thermo Fisher Scientific (2 mentions)
Dopamine hydrochloride, by Merck Group (2 mentions)
Diethyl ether, by Fujifilm (1 mentions)
Gapdh, by GeneTex (1 mentions)
Benserazide hydrochloride, by Merck Group (1 mentions)
Isoproterenol hydrochloride, by Merck Group (1 mentions)
α ketoglutaric acid sodium salt, by Merck Group (1 mentions)
Succinate glo jmjc demethylase hydroxylase assay kit, by Promega (1 mentions)
2 4 pyridinedicarboxylic acid 2 4 pdca, by Merck Group (1 mentions)
5 protocols using isoproterenol bitartrate
1
Synthesis and Characterization of Neuroactive Compounds
2
Synthesis and Characterization of Neuroactive Compounds
3
Isoproterenol-Induced Cardiac Remodeling
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Myocardial samples from mice subjected to continuous infusion of isoproterenol were used from a previously reported study (Gravning et al. 2013 (link)). Briefly, 6 months old male C57BL/6 mice were randomized to continuous subcutaneous infusion of isoproterenol bitartrate (50 mg/kg per day; Sigma-Aldrich) or vehicle for 14 days (vehicle, n = 9 and isoproterenol infusion, n = 9). Physical data from echocardiographic examination of these study groups have previously been reported (Gravning et al. 2013 (link)). Total RNA from myocardial biopsies from vehicle-infused (n = 6) and isoproterenol-infused (n = 5) mice were isolated and used for analysis of mRNA levels.
4
Eukaryotic Elongation Factor 2 Kinase Assay
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Reagent sources were as followings: propofol (Mylan N. V., Tokyo, Japan), diethyl ether (Wako Pure Chemical, Osaka, Japan), buprenorphine (Otsuka Pharmaceutical, Tokyo, Japan), isoproterenol bitartrate (Sigma-Aldrich, St. Louis, MO, USA), and pentobarbital (Sumitomo Dainippon Pharma, Tokyo, Japan).
Antibody sources were as followings: total-eEF2K (No. GTX107879) (Gene Tex, Irvine, CA, USA), phospho-eEF2K (at Ser366) (No. A0071) (Assay Biotech, Sunnyvale, CA, USA), phospho-eEF2 (at Thr56) (No. ADI-905-775-100) (Assay Designs, Ann Arbor, MI, USA), and GAPDH (No. GTX100118) (Gene Tex).
Antibody sources were as followings: total-eEF2K (No. GTX107879) (Gene Tex, Irvine, CA, USA), phospho-eEF2K (at Ser366) (No. A0071) (Assay Biotech, Sunnyvale, CA, USA), phospho-eEF2 (at Thr56) (No. ADI-905-775-100) (Assay Designs, Ann Arbor, MI, USA), and GAPDH (No. GTX100118) (Gene Tex).
5
Luminescent Enzymatic Assay for JmjC Demethylase Activity
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Ascorbate ((+)-sodium l-ascorbate), Fe(II) (ammonium iron(II) sulfate hexahydrate), and α-KG (α-ketoglutaric acid sodium salt) were from Sigma-Aldrich (St. Louis, MO). The inhibitor 2,4-pyridinedicarboxylic acid (2,4-PDCA) and the compounds (+)-isoproterenol (+)-bitartrate, benserazide hydrochloride, cafeic acid phenethyl ester, 7,8-dihydroxycoumarin, (-)-isoproterenol hydrochloride, and (+/-) taxifolin hydrate were also purchased from Sigma-Aldrich. The inhibitors 8-hydroxy-5-quinolinecarboxylic acid (IOX-1) and 5-chloro-2-[(E)-2[phenyl(pyridine-2-yl)methylidene] hydrazine-1-yl]pyridine (JIB 04) were purchased from TOCRIS (Bristol, UK). Daminozide and JHDM inhibitor III were purchased from EMD Millipore (Darmstadt, Germany). COSTAR 96-well half-area and 384-well low-volume white plates were purchased from Corning (Corning, NY).
The Succinate-Glo JmjC Demethylase/Hydroxylase Assay kit from Promega Corporation (Madison, WI) is composed of a succinate standard solution and components for two succinate detection reagents. Reagent I uses succinate as a substrate in a coupled enzyme reaction to produce ATP, and Reagent II detects ATP as light output from an ATP-dependent luciferase enzyme (Fig. 1).
The Succinate-Glo JmjC Demethylase/Hydroxylase Assay kit from Promega Corporation (Madison, WI) is composed of a succinate standard solution and components for two succinate detection reagents. Reagent I uses succinate as a substrate in a coupled enzyme reaction to produce ATP, and Reagent II detects ATP as light output from an ATP-dependent luciferase enzyme (Fig. 1).
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