Rabbit anti c myc n 262

Manufactured by Santa Cruz Biotechnology

Rabbit anti-c-Myc (N-262) is an antibody that recognizes the c-Myc protein. It is raised in rabbits against the N-terminal 262 amino acids of the c-Myc protein.

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Lab products found in correlation

Mouse anti gapdh 0411, by Santa Cruz Biotechnology (1 mentions) Rabbit anti β tubulin h 235, by Santa Cruz Biotechnology (1 mentions) Rabbit anti p21 h 164, by Santa Cruz Biotechnology (1 mentions) Rabbit anti mcl 1 h 260, by Santa Cruz Biotechnology (1 mentions) Mouse anti xiap, by BD (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Rabbit anti foxa2, by Merck Group (1 mentions) Donkey serum, by Jackson ImmunoResearch (1 mentions) Dmi6000b inverted fluorescence microscope, by Leica camera (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

2 protocols using rabbit anti c myc n 262

Protein Extraction and Western Blot Analysis

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For protein extraction, cells were washed with PBS and lysed using RIPA buffer (50 mM Tris-Cl, pH 7.4, 150 mM NaCl, 1% (w/v) sodium deoxycholate, 0.1% (v/v) SDS, 1% (v/v) Triton X-100, 2 mM EGTA, 2 mM EDTA, 50 mM NaF, 5 mM Na₂P₂O₇, 1 X complete protease inhibitor cocktail (Roche) and 0.1 M Sodium Orthovanadate). For PARP cleavage analysis, dead cells were collected by centrifugation and combined with live cell lysates. Lysates were sonicated, centrifuged, and the supernatant quantified using the BCA Protein Assay Kit (Pierce, Thermo Scientific) according to the manufacturer’s instructions. Proteins were subjected to Western blot analysis using the following antibodies: rabbit anti-Kpnβ1 (H-300, sc-11,367, Santa Cruz), rabbit anti-β-tubulin (H-235, sc-9104, Santa Cruz), rabbit anti-PARP1/2 antibody (H-250, sc-7150, Santa Cruz), mouse anti-GAPDH (0411, sc-47,724, Santa Cruz), rabbit anti-p21 (H-164, sc-756, Santa Cruz), rabbit anti-Mcl-1 (H-260, sc-20,679, Santa Cruz), mouse anti-cyclin D1 (HD11, sc-246, Santa Cruz), rabbit anti-c-Myc (N-262, sc-764, Santa Cruz), mouse anti-p53 (DO-7, M7001, DakoCytomation), mouse anti-XIAP (610,763, BD Biosciences), and rabbit anti-phospho-Histone H2AX (γH2AX, Ser139, 20E3, #9718, Cell Signal).

Chi R.P., van der Watt P., Wei W., Birrer M.J, & Leaner V.D. (2021). Inhibition of Kpnβ1 mediated nuclear import enhances cisplatin chemosensitivity in cervical cancer. BMC Cancer, 21, 106.

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Immunofluorescence Analysis of Cell Markers

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For cell immunofluorescence analysis, tissue culture plates were fixed for 20 minutes in 4% paraformaldehyde (Electron Microscopy Sciences) and washed in PBS with 0.1% Triton X-100 (Sigma). Tissues were blocked by 20 minute incubation at 4 degrees in PBS with 20% donkey serum (Jackson Immunoresearch) and 0.1% Triton X-100. Primary and secondary antibody staining were performed overnight at 4 degrees in PBS with 5% donkey serum and 0.1% Triton X-100, and after primary and secondary antibody staining, washing was performed with PBS with 0.1% Triton X-100. After staining, plates were washed and incubated with 1 μg/mL Hoechst 33342 (Life Technologies). Imaging was performed using a DMI 6000b inverted fluorescence microscope (Leica), and image analysis with the Leica AF6000 software package.
The following primary antibodies were used: goat anti-Foxa2 M-20, rabbit anti-RAR M-454, rabbit anti-cMyc N-262 (Santa Cruz Biotechnology), rabbit anti-Foxa2 (Millipore); goat anti-Sox17, mouse anti-Sox2, (R+D Systems); mouse anti-Hnf1β (BD Biosciences). AlexaFluor488 and AlexaFluor594 conjugates (Jackson Immunoresearch) were used for secondary detection.

Sherwood R.I., Hashimoto T., O'Donnell C.W., Lewis S., Barkal A.A., van Hoff J.P., Karun V., Jaakkola T, & Gifford D.K. (2014). Discovery of non-directional and directional pioneer transcription factors by modeling DNase profile magnitude and shape. Nature biotechnology, 32(2), 171-178.

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