Human beta 2 microglobulin elisa kit

Manufactured by Abcam

Sourced in United Kingdom

The Human beta-2 microglobulin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human beta-2 microglobulin levels in serum, plasma, and other biological fluids. The kit utilizes a specific antibody coated on a 96-well plate to capture the target analyte, which is then detected using a horseradish peroxidase (HRP)-conjugated detection antibody and a colorimetric substrate. The resulting color change is proportional to the concentration of human beta-2 microglobulin present in the sample.

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Lab products found in correlation

Uquantmicroplate spectrophotometer, by Agilent Technologies (1 mentions)

2 protocols using human beta 2 microglobulin elisa kit

Quantifying Serum Biomarkers

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b2M concentration was determined in triplicate using a commercial human beta-2 microglobulin ELISA Kit (Abcam) with a detection limit <6 pg/mL.
Albumin concentration was determined in triplicate with a 1 : 200 dilution using the commercial human albumin ELISA Kit (Alpha Diagnostic International) with intra-assay CV of 6.8 to 11.4% and interassay CV of 3.5 to 6.4%.

Rizzo R., Gabrielli L., Bortolotti D., Gentili V., Piccirilli G., Chiereghin A., Pavia C., Bolzani S., Guerra B., Simonazzi G., Cervi F., Capretti M.G., Fainardi E., Luca D.D., Landini M.P, & Lazzarotto T. (2016). Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection. Journal of Immunology Research, 2016, 3890306.

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Urinary Beta-2-Microglobulin Quantification

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Urinary concentrations of beta-2-microglobulin were measured using the human beta-2microglobulin ELISA Kit (Abcam, UK) in accordance with the manufacturer's instructions. Urine samples were initially stored at -70°C then thawed on ice prior to assay and quantified using the u-quant microplate spectrophotometer (Bio-Tek Instruments Inc., USA).

Dyck K.N., Bashir S., Horgan G.W, & Sneddon A.A. (2019). Regular crabmeat consumers do not show increased urinary cadmium or beta-2-microglobulin levels compared to non-crabmeat consumers. Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 52.

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