Intracellular anti foxp3

Manufactured by Thermo Fisher Scientific

Intracellular anti‐FoxP3 is a lab equipment product designed to detect the expression of the FoxP3 transcription factor in cells. FoxP3 is a key regulator of T-cell development and function, and its expression is used as a marker for regulatory T-cells. The Intracellular anti‐FoxP3 product is used to identify and quantify FoxP3-positive cells through flow cytometry or other immunodetection methods.

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Lab products found in correlation

C6 flow cytometer, by BD (2 mentions) Cflow software, by BD (2 mentions)

Close spelling variants of this product

Anti-Foxp3

2 protocols using intracellular anti foxp3

Multiparametric Analysis of Murine Splenocytes

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Splenocytes harvested from either naïve donor mice or BMT recipients were analyzed pretransplant or on D10, respectively. unless otherwise specified. Splenic cell suspensions were stained for surface T‐cell (CD4, CD8) and activation markers (CD25, CD28, CD62L, CD69) along with intracellular Tumor Necrosis Factor Alpha (TNFα) and Interferon Gamma (IFNγ) and then analyzed by flow cytometry using a four‐color C6 FlowCytometer (Accuri, Ann Arbor, Michigan) as previously described.²⁰ To analyze Forkhead Box P3 (FoxP3) expression, splenocytes were stained with anti‐CD4 and anti‐CD25, fixed and permeabilized, and then stained with intracellular anti‐FoxP3 (eBioscience, San Diego, California). All monoclonal antibodies were purchased from BD Biosciences Pharmingen (San Diego, California) or eBioscience. Irrelevant fluorochrome rat isotype immunoglobulins were used as negative controls. Data were analyzed using CFlow software (Accuri) as previously described.²⁰, ²⁵

Metheny L., Eid S., Wuttisarnwattana P., Auletta J.J., Liu C., Van Dervort A., Paez C., Lee Z., Wilson D., Lazarus H.M., Deans R., Vant Hof W., Ktena Y, & Cooke K.R. (2021). Human multipotent adult progenitor cells effectively reduce graft‐vs‐host disease while preserving graft‐vs‐leukemia activity. Stem Cells (Dayton, Ohio), 39(11), 1506-1519.

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Flow Cytometric Analysis of T-cell Activation

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Splenocytes harvested from either naïve donor mice or BMT recipients were analyzed pretransplant or on D10, respectively. unless otherwise specified. Splenic cell suspensions were stained for surface T-cell (CD4, CD8) and activation markers (CD25, CD28, CD62L, CD69) along with intracellular Tumor Necrosis Factor Alpha (TNFα) and Interferon Gamma (IFNγ) and then analyzed by flow cytometry using a four-color C6 FlowCytometer (Accuri, Ann Arbor, Michigan) as previously described.^{20 (link)} To analyze Forkhead Box P3 (FoxP3) expression, splenocytes were stained with anti-CD4 and anti-CD25, fixed and permeabilized, and then stained with intracellular anti-FoxP3 (eBioscience, San Diego, California). All monoclonal antibodies were purchased from BD Biosciences Pharmingen (San Diego, California) or eBioscience. Irrelevant fluorochrome rat isotype immunoglobulins were used as negative controls. Data were analyzed using CFlow software (Accuri) as previously described.^{20 (link),25 (link)}

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