Blood samples were collected in heparinized tubes via the ophthalmic vein. Inflammatory mediators, such as CCL4, IL-10, CXCL-1, TNF-α, IL-1β, IL-6, and IL-17A, were assessed by the Mice Cytokine Magnetic Bead Panel Kit (eBioscience, USA) following the manufacturer's protocol.
Mice cytokine magnetic bead panel kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Mice Cytokine Magnetic Bead Panel Kit is a laboratory tool designed for the detection and quantification of multiple cytokines in mouse biological samples. The kit utilizes magnetic beads coated with antibodies specific to different cytokines, allowing for the simultaneous measurement of these proteins in a single sample.
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4 protocols using mice cytokine magnetic bead panel kit
1
Cytokine Profiling in Murine Blood
2
Cytokine Quantification in Lung Samples
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IL-38 concentration was measured by a murine IL-38 ELISA kit (R&D Systems, California, USA, #DY2427-05) or the human IL-38 quanti cation ELISA kit (R&D Systems, California, USA, #DY9110-05), as appropriate. We also measured in ammatory mediators, including TNF-α, IL-1β, IL-6, IL-17A, and CXCL-1, in the BALF or lung homogenate, using the Mice Cytokine Magnetic Bead Panel Kit (eBioscience, USA) according to speci c instructions.
3
Murine and Human IL-38 ELISA Assay
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IL-38 concentration was measured by a murine IL-38 ELISA kit (R&D Systems, California, USA, #DY2427-05) or the human IL-38 quantification ELISA kit (R&D Systems, California, USA, #DY9110-05), as appropriate. We also measured inflammatory mediators, including TNF-α, IL-1β, IL-6, IL-17A, and CXCL-1, in the BALF or lung homogenate, using the Mice Cytokine Magnetic Bead Panel Kit (eBioscience, USA) according to specific instructions.
4
Inflammatory Mediator Profiling in Mice
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Blood samples were collected in heparinized tubes via the ophthalmic vein. In ammatory mediators, such as CCL4, CXCL-1, TNF-α, IL-1β, IL-6, and IL-17A, were assessed by the Mice Cytokine Magnetic Bead Panel Kit (eBioscience, USA) following the manufacturer's protocol.
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