Cpt 1b

Cells were washed with cold PBS and homogenized in lysis buffer containing protease inhibitors (keyGEN, Nanjing, China) on ice. After centrifugation, the supernatant containing protein was collected. Total protein and 5 × SDS loading buffer were mixed and boiled at 100 °C for 5 min. Samples were separated by electrophoresis on 10% SDS-polyacrylamide gel and transferred onto polyvinylidene fluoride membranes, after which the membranes were blocked for 1 h at room temperature with 5% skim milk supplemented with 0.1% Tween 20 (TBST). Each membrane was then first incubated overnight with a primary antibody at 4 °C and then with a secondary antibody for 60 min at room temperature. Immunoreactive bands were visualized using a chemiluminescence (ECL) detection system or LI-COR Odyssey infrared imaging system. Primary antibodies are listed below: PITPNC1 (Sigma, Saint Louis, MO, USA), CPT1B, p-AKT (Ser473), MMP9, Cleaved Caspase 3 (Cell Signaling Technology, Danvers, MA, USA), SREBP1 (Novus Biological), CD36, BCL2, BAX, E-cadherin, Vimentin, PPARγ, PPARα/β, Ki67 (Abcam, Cambridge, MA, USA), Histone H3, β-actin (Proteintech, Chicago, USA).