Anti mouse albumin elisa kit

Serum IgG subtypes (IgG1 and IgG2a) were measured by ELISA using the following coating antibodies: purified anti-mouse IgG1 and IgG2a and detection antibodies: biotin anti-mouse IgG1 and IgG2a (BD Pharmingen, San Jose, CA). Following Streptavidin-HRP (Sigma) incubation, the ELISA was developed with TMB substrate (Dako). The optical density (OD) for each well with a microplate reader set to 450 nm. MCP-1 and s100a8/9 levels were measured via ELISA according to the manufacturer's instruction (R&D). Total serum IgG and anti-dsDNA and anti-RNP Ab levels were quantified by ELISA using commercially available kits (Alpha Diagnostic International, San Antonio, TX), following manufacturer's protocols. An anti-mouse albumin ELISA kit was used to measure urine proteinuria (Bethyl labs).

An anti-mouse albumin ELISA kit was used to measure urine protein as per manufacturer's protocol (Bethyl Laboratories). Serum cytokine levels were determined using ELISA kits as per manufacturer's protocol: anti-mIFNγ and anti-mIL-17 (BD Biosciences), anti-mIL-10 (eBioscience) and anti-mTNFa (R&Dsystems). The IFN-β level in media supernatant was determined as previously described (36 (link)).