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Anti mouse albumin elisa kit

Manufactured by Fortis Life Sciences

The Anti-mouse Albumin ELISA Kit is a quantitative immunoassay designed for the measurement of mouse albumin in biological samples. The kit utilizes the sandwich ELISA technique to detect and quantify mouse albumin levels.

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2 protocols using anti mouse albumin elisa kit

1

Quantifying Serum Immunoglobulins and Biomarkers

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Serum IgG subtypes (IgG1 and IgG2a) were measured by ELISA using the following coating antibodies: purified anti-mouse IgG1 and IgG2a and detection antibodies: biotin anti-mouse IgG1 and IgG2a (BD Pharmingen, San Jose, CA). Following Streptavidin-HRP (Sigma) incubation, the ELISA was developed with TMB substrate (Dako). The optical density (OD) for each well with a microplate reader set to 450 nm. MCP-1 and s100a8/9 levels were measured via ELISA according to the manufacturer's instruction (R&D). Total serum IgG and anti-dsDNA and anti-RNP Ab levels were quantified by ELISA using commercially available kits (Alpha Diagnostic International, San Antonio, TX), following manufacturer's protocols. An anti-mouse albumin ELISA kit was used to measure urine proteinuria (Bethyl labs).
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2

Quantitative Protein and Cytokine Analysis

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An anti-mouse albumin ELISA kit was used to measure urine protein as per manufacturer's protocol (Bethyl Laboratories). Serum cytokine levels were determined using ELISA kits as per manufacturer's protocol: anti-mIFNγ and anti-mIL-17 (BD Biosciences), anti-mIL-10 (eBioscience) and anti-mTNFa (R&Dsystems). The IFN-β level in media supernatant was determined as previously described (36 (link)).
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