The forced swim test was performed as previously described (Turner et al. 2010). Mice were placed in a Plexiglass cylinder filled with water at room temperature for 6 minutes. Video tape recordings were scored by an experimenter blinded to the experimental conditions for time spent immobile (i.e. movement necessary for floating only) during the full 6 minute test. Trunk blood was collected from each mouse 20 minutes after the start of the forced swim test. Plasma was separated by centrifugation and stored at −20°C until assayed for coriticosterone using a commercially available radioimmunoassay kit (ICN Biomedicals Inc., Cleveland, OH). Intra-assay coefficient variation was < 20%.
Radioimmunoassay kit
Manufactured by ICN Biomedicals
Sourced in United States
The Radioimmunoassay kit is a laboratory equipment used for the quantitative analysis of various substances, including hormones, proteins, and other biomolecules. It utilizes the principles of radioimmunoassay, a sensitive analytical technique that employs radioactive tracer molecules and specific antibodies to measure the concentration of target analytes in a sample.
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Lab products found in correlation
6 protocols using radioimmunoassay kit
1
Forced Swim Test for Mice
2
Plasma Corticosterone Determination
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Trunk blood was collected in tubes containing 10 μg EDTA, centrifuged at 3600 rpm for 8 min, and 50 μL of plasma was then collected for determination of corticosterone levels. Plasma was immediately frozen at –80oC until analyses. Corticosterone levels were measured using a commercial radioimmunoassay kit (ICN Biomedicals, CA, USA, Cat .no 07120002). Inter-assay variability was avoided by assaying all samples (in duplicate) within a single run.
3
Burn-Induced Metabolic Alterations
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The rats were fasted for 12 h before burn or sham injury. Eight animals were killed, and blood was drawn from the abdominal aorta. Fasting blood glucose levels were determined before injury (d0) and at days 1, 4, 7, and 14 after injury (fasted for 8 h before blood glucose measurement). Fasting blood glucose levels were determined using an Accu-chek Active Blood Glucose Monitor (Hoffmann-La Roche, Basel, Switzerland). Serum total testosterone was measured in duplicate using a radioimmunoassay kit (ICN-Biomedicals, Irvine, Calif). Serum and TA muscle cell lysate IGF-1 were assayed using commercially available immunoassay kits using the xMAP technology (Linco Research, St Charles, Mo).
4
Plasma Corticosterone Measurement
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At the time of decapitation, trunk blood from all of the animals was collected in tubes containing 10 μg EDTA. Samples were centrifuged (3000g for 8 min), and the plasma removed and stored in aliquots at − 80 °C for later corticosterone determination with commercially available radioimmunoassay kits (ICN Biomedicals, CA, USA). Samples were assayed in duplicate within a single run to control for inter-assay variability; the intra-assay variability was less than 10%.
5
Plasma Corticosterone and Cytokine Assay
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At the time of decapitation, trunk blood from all of the animals, including those in the behavioural testing-naive control group, was collected in tubes containing 10 μg EDTA. Samples were centrifuged (3000g for 8 min) and the plasma removed and stored in aliquots at −80 °C for later corticosterone determination with commercially available radioimmunoassay kits (ICN Biomedicals, CA, USA). Samples were assayed in duplicate within a single run to control for inter-assay variability; the intra-assay variability was less than 10%. Separate plasma aliquots were used for the cytokine determinations.
6
Baseline Plasma Corticosterone Levels
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All animals were sacrificed at the age of 21-25 weeks by decapitation between 8.00 and 11.00 hrs in the morning, and trunk blood was collected within 30s after the animal's removal from the cage. Baseline plasma corticosterone levels -without applying any acute stress or intervention before taking the samples -were determined using commercially available radioimmunoassay kits (ICN Biomedicals, Eschwege, Germany) as described earlier [49] .
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