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Omni map anti rabbit hrp conjugated secondary antibody multimer hrp

Manufactured by Roche
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The Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) is a laboratory reagent designed for use in immunohistochemical and western blotting applications. It is a horseradish peroxidase (HRP)-labeled secondary antibody that binds to primary rabbit antibodies, enabling the detection of target proteins.

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Lab products found in correlation

Discovery chromomap dab kit, by Roche (3 mentions) Discovery ultra antibody block, by Roche (3 mentions) Anti phospho stat1 tyr701 58d6, by Cell Signaling Technology (3 mentions) Ventana discovery ultra, by Roche (2 mentions) Anti cd68 clone kp1, by Abcam (2 mentions) Discovery ultra, by Roche (1 mentions)

Close spelling variants of this product

HRP-conjugated secondary antibody (5 citations) Omni Map anti-Rabbit (3 citations)

3 protocols using omni map anti rabbit hrp conjugated secondary antibody multimer hrp

1

Immunohistochemical Analysis of STAT1 and STAT3 Phosphorylation

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Immunohistochemistry staining was performed with a Discovery Ultra instrument (Roche), with the staining module of RUO Discovery Multimer V2 (V0.00.0083). Slides were first incubated in blocking agent containing 2% BSA PBS, and Discovery Ultra antibody block (Cat # 760-4204) (Roche). Slides were incubated with primary antibodies for 60 minutes at 37°C. The following primary antibodies were used: anti-phospho-STAT1 (Tyr701) 58D6 (Cell Signaling) and anti-phospho-STAT3 (Tyr 705) D3A7 XP (Cell Signaling) at 1:100 dilution in blocking agent. The slides were then incubated with Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) (Cat # 760-4311) (Roche) for 32 minutes. Positive signals were detected with the Discovery ChromoMap DAB Kit (Cat #760-159) (Roche).
Gruber C.N., Calis J.J., Buta S., Evrony G., Martin J.C., Uhl S.A., Caron R., Jarchin L., Dunkin D., Phelps R., Webb B.D., Saland J.M., Merad M., Orange J.S., Mace E.M., Rosenberg B.R., Gelb B.D, & Bogunovic D. (2020). Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity, 53(3), 672-684.e11.
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2

Immunohistochemical Analysis of Phospho-STAT1 and CD68

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Immunohistochemistry staining was performed using a Discovery Ultra Ventana instrument (Roche), with the staining module of RUO Discovery Multimer V2 (V0.00.0083). Slides were first incubated in blocking agent containing 2% BSA PBS, and Discovery Ultra antibody block (Cat # 760–4204) (Roche). Slides were incubated with primary antibodies for 60 minutes at 37°C. The following primary antibodies were used: anti-phospho-STAT1 (Tyr701) 58D6 (Cell Signaling) and anti-CD68 Clone Kp1 (Abcam) at recommended dilution in blocking agent. The slides were then incubated with Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) (Cat # 760–4311) (Roche) for 32 minutes. Positive signals were detected with the Discovery ChromoMap DAB Kit (Cat #760–159) (Roche).
Martin-Fernandez M., García-Morato M.B., Gruber C., Loza S.M., Malik M.N., Alsohime F., Alakeel A., Valdez R., Buta S., Buda G., Marti M.A., Larralde M., Boisson B., Rodriguez M.F., Qiu X., Chrabieh M., Ayed M.A., Muhsen S.A., Desai J.V., Ferre E.M., Rosenzweig S.D., Amador-Borrero B., Bravo-Gallego L.Y., Olmer R., Merkert S., Bret M., Sood A.K., Al-rabiaah A., Temsah M.H., Halwani R., Hernandez M., Pessler F., Casanova J.L., Bustamante J., Lionakis M.S, & Bogunovic D. (2020). Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions. Cell reports, 31(6), 107633.
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3

Immunohistochemical Analysis of Phospho-STAT1 and CD68

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry staining was performed using a Discovery Ultra Ventana instrument (Roche), with the staining module of RUO Discovery Multimer V2 (V0.00.0083). Slides were first incubated in blocking agent containing 2% BSA PBS, and Discovery Ultra antibody block (Cat # 760–4204) (Roche). Slides were incubated with primary antibodies for 60 minutes at 37°C. The following primary antibodies were used: anti-phospho-STAT1 (Tyr701) 58D6 (Cell Signaling) and anti-CD68 Clone Kp1 (Abcam) at recommended dilution in blocking agent. The slides were then incubated with Omni Map anti-rabbit HRP-conjugated secondary antibody (Multimer HRP) (Cat # 760–4311) (Roche) for 32 minutes. Positive signals were detected with the Discovery ChromoMap DAB Kit (Cat #760–159) (Roche).
Martin-Fernandez M., García-Morato M.B., Gruber C., Loza S.M., Malik M.N., Alsohime F., Alakeel A., Valdez R., Buta S., Buda G., Marti M.A., Larralde M., Boisson B., Rodriguez M.F., Qiu X., Chrabieh M., Ayed M.A., Muhsen S.A., Desai J.V., Ferre E.M., Rosenzweig S.D., Amador-Borrero B., Bravo-Gallego L.Y., Olmer R., Merkert S., Bret M., Sood A.K., Al-rabiaah A., Temsah M.H., Halwani R., Hernandez M., Pessler F., Casanova J.L., Bustamante J., Lionakis M.S, & Bogunovic D. (2020). Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions. Cell reports, 31(6), 107633.
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