Blood vessel density was assessed using co‐immunostaining for α‐smooth muscle actin (α‐SMA) and cluster of differentiation factor 31 (CD31) as previously described.23 Co‐staining was used due to the fact that immature blood vessels such as those in remodeling hypertrophic scars may not have full pericyte coverage that could be identified with α‐SMA alone.25 Biopsies taken at baseline representing normal skin (n = 4 Dc, n = 4 Yk) and at day 98 representing HTS (n = 4 Dc, n = 4 Yk) were sectioned and stained using primary antibodies for α‐SMA (Abcam, ab7817, 1:250, mouse) and CD31 (Biorbyt, orb10314, 1:500, rabbit) and secondary antibodies (goat anti‐mouse‐CY3 and goat anti‐rabbit‐CY5, Abcam). Two photos of the papillary dermis were taken at 10× magnification for each biopsy. Blood vessel density was calculated by counting the number of vessels and dividing by the area. Secondary antibody controls were stained in parallel and did not show nonspecific staining.
Orb10314
Manufactured by Biorbyt
The Orb10314 is a laboratory equipment product from Biorbyt. It is designed for general laboratory use. No additional details can be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using orb10314
1
Quantifying Blood Vessel Density in Hypertrophic Scars
2
Quantifying Vascular Density in Infarct Zones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sections were incubated with anti-cluster of differentiation 31 (CD31) primary antibody (orb10314; Biorbyt, 1:100) overnight at 4 °C. After washing, slides were incubated with the secondary antibody (orb688925; Biorbyt, 1:150) for 2 h at 37 °C. Afterward, the slides were washed and incubated with DAPI (Sigma-Aldrich, D9542) for nuclear staining at RT. Vascular density was quantified by the counting of stained capillary structures in 5 randomly high-power fields (HPF) per sample in the infarct border zone.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!