Four-week-old juvenile wild-type ferrets (n = 15 per experimental group) were anesthetized with a mixture of ketamine and xylazine injected subcutaneously and were i.t. administered AAV2.5T-SP183-fCFTRΔR at a dose of 1 × 1013 DRP/kg body weight (BW). Four weeks later, anesthetized ferrets were administered 1 × 1013 DRP/kg BW AAV2.5T-SP183-gLuc. The ferrets were dosed using a microsprayer aerosolizer (PennCentury, model IA-1B). The AAV2.5T vector inoculum contained doxorubicin at a concentration of 200 μM, and the administered volume was normalized to ferret BW at 2 mL/kg BW.
Daily administration of immunosuppressants started 2 days before the first vector dose and continued until the dosing day of the second vector dose (30 days of total dosing). Specifically, the cyclosporine (1 mg/kg BW, Novartis) and MP (2 mg/kg BW, Pfizer) were administered intraperitoneally, and azathioprine (2 mg/kg BW, Wedgewood Pharmacy) was given orally. The antibiotic Baytril (10 mg/kg BW, Bayer) was injected daily subcutaneously to prevent bacterial infection in the immunosuppressed ferrets.
Daily administration of immunosuppressants started 2 days before the first vector dose and continued until the dosing day of the second vector dose (30 days of total dosing). Specifically, the cyclosporine (1 mg/kg BW, Novartis) and MP (2 mg/kg BW, Pfizer) were administered intraperitoneally, and azathioprine (2 mg/kg BW, Wedgewood Pharmacy) was given orally. The antibiotic Baytril (10 mg/kg BW, Bayer) was injected daily subcutaneously to prevent bacterial infection in the immunosuppressed ferrets.