Mibefradil m5441

Rabbit IgG (sc-2027), rabbit anti-human CatSper 1 (H300) (sc-33153), rabbit anti-CatSper 1 (Santa Cruz, sc-33153), rabbit anti-human CCR 6 (H-81) (sc-5623), goat anti-human CCR-6 (N-19) (sc-9695), mouse anti-human CCR6 (R&D MAB195), and rabbit anti-human DEFB1 (FL-68) (sc-20797) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant human active DEFB1 (ab50048) was purchased from Abcam (Cambridge, MA). CCL20 (360-MP-025/CF) was purchased from R & D Systems. Quinn’s human tubal fluid (HTF) (ART-1020) media was purchased from SAGE Media. Mouse monoclonal anti-β-actin (1:5000, A1978), CatSper1 ion channel activator Progesterone (P8783), CatSper1 ion channel inhibitor Mibefradil (M5441) and NNC 55-0396 hydrate (N0287) were purchased from Sigma-Aldrich Co (St Louis, MO). Discontinuous density gradient Percoll (17-0891-01) was purchased from GE Healthcare Life Sciences (Pittsburg, PA).

STAT5 inhibitor pimozide (P1793) was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Imipramine HCl, ABT-737, ABT-263, ABT-199 were purchased from Selleck Chemicals (Houston, TX, USA) and used interchangeably in these studies. Initial experiments began with ABT-737 and as additional more clinically relevant forms were available the studies moved to ABT-263 and finally to ABT-199. These BH3 mimetics have identical function in vitro on cultured AML cells. AZD 8055 was purchased from Selleck Chemicals (Houston, TX, USA). Imipramine blue and gentian violet were provided by the Jack Arbiser lab. Mibefradil (M5441) and fluoxetine (F132) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA) as indicated. DPI was obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA) (D2926).