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Native ferritin

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Native ferritin is a naturally occurring protein complex found in various organisms. Its core function is to store and transport iron within cells. The complex is composed of 24 subunits that form a shell-like structure capable of storing up to 4,500 iron atoms.

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Lab products found in correlation

Il 18, by Abcam (2 mentions) Il 1b, by R&D Systems (2 mentions) Rbc lysis buffer, by BioLegend (2 mentions) Ficoll paque plus, by GE Healthcare (2 mentions) Sepmate tube, by STEMCELL (2 mentions) Interleukin 6 (il 6), by R&D Systems (2 mentions)

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Ferritin (27 citations)

2 protocols using native ferritin

1

PBMCs Characterization and Stimulation

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Mononuclear cells were purified from buffy coat by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare) in SepMate tubes (STEMCELL Technology). Crude PBMCs were treated with RBC lysis buffer (BioLegend) for 5 mins to remove residual red blood cells followed by 3 PBS washes. 2.5×105 PBMCs were resuspended in 250 μL of RPMI with 20 μL of serum from healthy donors or sJIA patients. Cells were processed for CyTOF analysis after overnight incubation. For in vitro PBMC stimulations, 1×106 PBMCs were resuspended in 1 mL RPMI and treated with 50 ng/mL IL-6 (R&D), 25 ng/mL IL-1B (R&D), 10 ng/mL IL-18, 5 μg/mL native ferritin (abcam), or 5 μg/mL recombinant ferritin. All human subject research was approved by Stanford Institutional Review Board (IRB).
Cheung P., Schaffert S., Chang S.E., Dvorak M., Donato M., Macaubas C., Foecke M.H., Li T.M., Zhang L., Coan J.P., Schulert G.S., Grom A.A., Henderson L.A., Nigrovic P.A., Elias J.E., Gozani O., Mellins E.D., Khatri P., Utz P.J, & Kuo A.J. (2021). REPRESSION OF CTSG, ELANE, AND PRTN3-MEDIATED HISTONE H3 PROTEOLYTIC CLEAVAGE PROMOTES MONOCYTE-TO-MACROPHAGE DIFFERENTIATION. Nature immunology, 22(6), 711-722.
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2

PBMCs Characterization and Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cells were purified from buffy coat by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare) in SepMate tubes (STEMCELL Technology). Crude PBMCs were treated with RBC lysis buffer (BioLegend) for 5 mins to remove residual red blood cells followed by 3 PBS washes. 2.5×105 PBMCs were resuspended in 250 μL of RPMI with 20 μL of serum from healthy donors or sJIA patients. Cells were processed for CyTOF analysis after overnight incubation. For in vitro PBMC stimulations, 1×106 PBMCs were resuspended in 1 mL RPMI and treated with 50 ng/mL IL-6 (R&D), 25 ng/mL IL-1B (R&D), 10 ng/mL IL-18, 5 μg/mL native ferritin (abcam), or 5 μg/mL recombinant ferritin. All human subject research was approved by Stanford Institutional Review Board (IRB).
Cheung P., Schaffert S., Chang S.E., Dvorak M., Donato M., Macaubas C., Foecke M.H., Li T.M., Zhang L., Coan J.P., Schulert G.S., Grom A.A., Henderson L.A., Nigrovic P.A., Elias J.E., Gozani O., Mellins E.D., Khatri P., Utz P.J, & Kuo A.J. (2021). REPRESSION OF CTSG, ELANE, AND PRTN3-MEDIATED HISTONE H3 PROTEOLYTIC CLEAVAGE PROMOTES MONOCYTE-TO-MACROPHAGE DIFFERENTIATION. Nature immunology, 22(6), 711-722.
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