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Anti larg mouse monoclonal

Manufactured by Merck Group
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The Anti-LARG mouse monoclonal is a laboratory reagent used for the detection and analysis of the LARG protein in biological samples. It is a mouse-derived monoclonal antibody specific to the LARG protein.

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Lab products found in correlation

M per mammalian protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Bca protein assay, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor, by Merck Group (2 mentions) Chemidoc touch imaging system, by Bio-Rad (1 mentions) Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti phospho cofilin, by Abcam (1 mentions) Anti phospho p70 s6 kinase, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti gapdh, by Cell Signaling Technology (1 mentions) Ge amersham imager 600, by GE Healthcare (1 mentions) Amersham imager, by Cytiva (1 mentions)

Close spelling variants of this product

Anti-LARG (2 citations) Monoclonal mouse (2 citations)

2 protocols using anti larg mouse monoclonal

1

Western Blot Analysis of Cell Signaling

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Protein was extracted from whole cell lysates using the Mammalian Protein Extraction Reagent M-PER (Thermo Scientific Pierce, Rockford, IL) supplemented with a cocktail of protease and phosphatase inhibitors (Sigma) and quantified through the BCA protein assay (Thermo Scientific Pierce). Lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. We probed for anti-TSHR (4C1) mouse monoclonal (Thermo Fisher Scientific) at 1:1000, anti-Gα12 (N3C3) rabbit polyclonal (GeneTex, Irvine, CA) at 1:1000, anti-Gα13 rabbit polyclonal (NewEast Bioscience, Malvern, PA) at 1:1000, anti-PTEN (6H2.1) mouse monoclonal (Cascade Bioscience, Winchester, MA) at 1:1000, anti-LARG mouse monoclonal (EMD Millipore, Temecula, CA) at 1:10000, anti Phospho-AKT (Ser473) rabbit polyclonal (Cell Signaling #9271L) at 1:1000, anti-Phospho-p70 S6 Kinase (Thr389) (1A5) mouse monoclonal (Cell Signaling #9206S) at 1:1000, anti Phospho-S6 ribosomal protein (S235/236) rabbit monoclonal (Cell Signaling #4858S) at 1:1000, anti-Phospho-cofilin (Abcam #ab12866) at 1:1000 and anti-GAPDH rabbit monoclonal (Cell Signaling #2118) at 1:20000 dilution. Blots were scanned digitally using the ChemiDoc™ touch imaging system (Bio-Rad, Hercules, CA). Densitometry was performed using ImageJ software.
Feng F., Han H., Wu S, & Wang H. (2021). Crosstalk Between Abnormal TSHR Signaling Activation and PTEN/PI3K in the Dedifferentiation of Thyroid Cancer Cells. Frontiers in Oncology, 11, 718578.
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2

Western Blot Protocol for Protein Analysis

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Protein was extracted from whole cell lysates using the Mammalian Protein Extraction Reagent M-PER (Thermo Scientific Pierce, Rockford, IL) supplemented with a cocktail of protease and phosphatase inhibitors (Sigma) and quantified through the BCA protein assay (Thermo Scientific Pierce). Lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. We probed for rabbit anti-NIS at 1:4000, anti-PTEN (6H2.1) mouse monoclonal (Cascade Bioscience, Winchester, MA) at 1:1000, anti-LARG mouse monoclonal (EMD Millipore, Temecula, CA) at 1:5000, and anti-GAPDH rabbit monoclonal (Cell Signaling #2118) at 1:20000 dilution. Blots were scanned digitally using the GE Amersham Imager 600 (GE Healthcare Life Science, Chicago, IL). Densitometry was performed using ImageJ software. Uncropped versions of cropped immunoblots are included in Supplementary Fig. S1A-G.
Feng F., Yehia L., Ni Y., Chang Y.S., Jhiang S.M, & Eng C. (2018). A non-pump function of sodium iodide symporter in thyroid cancer via crosstalk with PTEN signaling. Cancer research, 78(21), 6121-6133.
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