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Ad myc bap1 c91s

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Ad-Myc-BAP1(C91S) is a recombinant protein produced by SignaGen Laboratories. It contains the Myc-tagged BAP1 protein with the C91S mutation. The core function of this product is to serve as a tool for research and experimental purposes.

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Lab products found in correlation

Sirna oligonucleotides, by Qiagen (3 mentions) Ad bap1, by SignaGen (3 mentions) Hiperfect, by Qiagen (3 mentions) Ad myc bap1 w, by SignaGen (2 mentions) Ad myc bap1, by SignaGen (2 mentions)

Spelling variants of this product

Ad-BAP1 (3 citations) Ad-Myc-BAP1 (2 citations)

3 protocols using ad myc bap1 c91s

1

siRNA Knockdown and Adenoviral Overexpression of BAP1 and IP3R3

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siRNA oligonucleotides were obtained from Qiagen. GeneSoluton siRNAs targeting four different BAP1 mRNAs: Hs_BAP1_1, cat.no: SI00066696; Hs_BAP1_2, cat.no: SI00066703; Hs_BAP1_3, cat.no: SI00066710; Hs_BAP1_5, cat.no: SI03036390. GeneSoluton siRNAs targeting four different IP3R3 mRNAs (ITPR3): Hs_ITPR3_1, cat.no: SI00034580; Hs_ ITPR3_2, cat.no: SI00034587; Hs_ ITPR3_3, cat.no: SI00034594; Hs_ ITPR3_4, cat.no: SI00034601. Negative control siRNA, cat.no: 1027280. Transfection was performed with HiPerfect (Qiagen), using siRNAs at 10 nM final concentrations in 0.1% FBS medium for 24 hours. Unless otherwise specified in the Figures, a pool of the 4 different siRNAs, listed above, was used to silence BAP1 or IP3R3.
Adenoviruses expressing BAP1 and GFP were purchased from SignaGen Laboratories (Ad-BAP1, cat. no. SL175127; Ad-GFP, cat. no. SL100708). Customized adenoviruses expressing Ad-Myc-BAP1, Ad-Myc-BAP1(C91S), Ad-Myc-BAP1(W) and Ad-Myc-BAP1(L), were produced by SignaGen Laboratories using their Ad.MAX™ System. Expression plasmids for Flag-HA-BAP1, Myc-BAP1, Myc-BAP1(W), Myc-BAP1(L) and Myc-BAP1(C91S) were produced by Blue Heron Biotech. For BAP1(W), BAP1(L) and BAP1(C91S) the following nucleotide sequences were used:
Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris L.K., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass H.I., Affar E.B., Yang H., Pinton P, & Carbone M. (2017). BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature, 546(7659), 549-553.
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2

Targeting BAP1 with siRNA and Adenoviral Constructs

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siRNA oligonucleotides targeting four different BAP1 mRNAs and one negative control siRNA were obtained from Qiagen (Germantown, MD, USA) (GeneSoluton siRNA: Hs_BAP1_1, Cat. No.: SI00066696; Hs_BAP1_2, Cat. No.: SI00066703; Hs_BAP1_3, Cat. No.: SI00066710; Hs_BAP1_5, Cat. No.: SI03036390; AllStars Negative control siRNA, Cat. No.: 1027280). Tranfection was performed with HiPerfect (Qiagen), using a 10 nM final concentration of siRNA in 10 % FBS medium for 24 h.
The adenoviruses expressing BAP1 and GFP were purchased from SignaGen Laboratories (Rockville, MD, USA) (Ad-BAP1, Cat. No. SL175127; Ad-GFP, Cat. No. SL100708). A customized adenovirus expressing Ad-Myc-BAP1(C91S) was produced by SignaGen Laboratories using their Ad.MAX System.
Bononi A., Yang H., Giorgi C., Patergnani S., Pellegrini L., Su M., Xie G., Signorato V., Pastorino S., Morris P., Sakamoto G., Kuchay S., Gaudino G., Pass H.I., Napolitano A., Pinton P., Jia W, & Carbone M. (2017). Germline BAP1 mutations induce a Warburg effect. Cell Death and Differentiation, 24(10), 1694-1704.
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3

siRNA Knockdown and Adenoviral Overexpression of BAP1 and IP3R3

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siRNA oligonucleotides were obtained from Qiagen. GeneSoluton siRNAs targeting four different BAP1 mRNAs: Hs_BAP1_1, cat.no: SI00066696; Hs_BAP1_2, cat.no: SI00066703; Hs_BAP1_3, cat.no: SI00066710; Hs_BAP1_5, cat.no: SI03036390. GeneSoluton siRNAs targeting four different IP3R3 mRNAs (ITPR3): Hs_ITPR3_1, cat.no: SI00034580; Hs_ ITPR3_2, cat.no: SI00034587; Hs_ ITPR3_3, cat.no: SI00034594; Hs_ ITPR3_4, cat.no: SI00034601. Negative control siRNA, cat.no: 1027280. Transfection was performed with HiPerfect (Qiagen), using siRNAs at 10 nM final concentrations in 0.1% FBS medium for 24 hours. Unless otherwise specified in the Figures, a pool of the 4 different siRNAs, listed above, was used to silence BAP1 or IP3R3.
Adenoviruses expressing BAP1 and GFP were purchased from SignaGen Laboratories (Ad-BAP1, cat. no. SL175127; Ad-GFP, cat. no. SL100708). Customized adenoviruses expressing Ad-Myc-BAP1, Ad-Myc-BAP1(C91S), Ad-Myc-BAP1(W) and Ad-Myc-BAP1(L), were produced by SignaGen Laboratories using their Ad.MAX™ System. Expression plasmids for Flag-HA-BAP1, Myc-BAP1, Myc-BAP1(W), Myc-BAP1(L) and Myc-BAP1(C91S) were produced by Blue Heron Biotech. For BAP1(W), BAP1(L) and BAP1(C91S) the following nucleotide sequences were used:
Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris L.K., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass H.I., Affar E.B., Yang H., Pinton P, & Carbone M. (2017). BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature, 546(7659), 549-553.
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