Total RNA was extracted from 100 mg of epididymal WAT using the RNeasy Lipid Tissue Mini Kit (Qiagen, Tokyo, Japan). TaqMan Universal PCR Master Mix (Applied Biosystems, Tokyo, Japan) and Assay-on-Demand, Gene Expression Products (Mn00456425_m1 for adiponectin, Mn00446190_m1 for IL-6, Mn00443258_m1 for TNFα, Mn00441242_m1 for MCP1, and Mm04277571_s1 for 18S RNA, all from Applied Biosystems, Tokyo, Japan) were used for quantitative RT-PCR analysis of adiponectin, MCP1, TNFα, and 18S RNA expression in epididymal WAT. Amplifications were carried out using an ABI Prism 7000 RT-PCR sequence detection system (Applied Biosystems, Tokyo, Japan).
Abi prism 7000 rt pcr sequence detection system
Manufactured by Thermo Fisher Scientific
Sourced in Japan
The ABI Prism 7000 RT-PCR sequence detection system is a real-time PCR instrument. It is designed to perform quantitative and qualitative analysis of DNA and RNA samples.
Automatically generated - may contain errors
2 protocols using abi prism 7000 rt pcr sequence detection system
1
Adipokine Expression in Epididymal WAT
2
Quantitative RT-PCR of Liver Lipid Genes
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Total RNA was extracted from 100 mg of liver using the RNeasy Lipid Tissue Mini Kit (Qiagen, Tokyo, Japan). TaqMan Universal PCR Master Mix (Applied Biosystems, Tokyo, Japan) and Assay-on-Demand, Gene Expression Products (Mn01304289_m1 for ACC1, Mn00662319_m1 for FAS, Mn00550438_m1 for CPT1a, Mn00443579_m1 for ACO1, Mn00441242_m1 for MCP1, Mn00443258_m1 for TNFα, and Mm04277571_s1 for 18S rRNA, all from Applied Biosystems, Tokyo, Japan) were used for quantitative RT-PCR analysis of ACC1, FAS, CPT1a, ACO1, MCP1, TNFα, and 18S RNA expression in the liver. Amplifications were carried out using an ABI Prism 7000 RT-PCR sequence detection system (Applied Biosystems, Tokyo, Japan).
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