Leukocytes (~1×106 cells/tube) were stained with DAPI (cell viability), anti-CD45-Pacific Blue, anti-F4/80-FITC, anti-CD11b-PercP Cy5.5, anti-Ly6G-APC, anti-CXCR2-PE (all R&D Systems), anti-CD62L-FITC, anti-GRK2-FITC, anti-Cytokeratin (C-11)-FITC (Abcam), anti-IL-33-PE (R&D Systems) and isotype controls (BD Biosciences), following the manufacturer’s protocols. IL-33 production by CD45− Cytokeratin+ epT cells was determined after stimulation with PMA (50 ng/ml) and ionomycin (1 μg/ml) in the presence of GolgiStop™ and permeabilization of the cells (Cytofix/Cytoperm, BD Biosciences). Cells were acquired using a Beckman Coulter CyAn™ ADP (Beckman Coulter, USA). Gating strategy (Supplementary Figure S5 online ) and analysis were performed using the FlowJo® software (treeStar Software, USA).
Anti cd11b percp cy5
Manufactured by R&D Systems
Anti-CD11b-PercP Cy5.5 is a fluorescently-labeled antibody that binds to the CD11b cell surface antigen. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used in flow cytometry applications to identify and quantify CD11b-positive cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Isotype control, by BD (2 mentions)
Anti cd45 pacific blue, by R&D Systems (2 mentions)
Anti f4 80 fitc, by R&D Systems (2 mentions)
Anti ly6g apc, by R&D Systems (2 mentions)
Anti cxcr2 pe, by R&D Systems (2 mentions)
Anti cd62l fitc, by Abcam (2 mentions)
Anti grk2 fitc, by Abcam (2 mentions)
Anti cytokeratin c 11 fitc, by Abcam (2 mentions)
Anti il 33 pe, by R&D Systems (2 mentions)
Cyan adp, by Beckman Coulter (2 mentions)
Cytofix cytoperm, by BD (2 mentions)
2 protocols using anti cd11b percp cy5
1
IL-33 Production in Epidermal T Cells
2
IL-33 Production in Epidermal T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Leukocytes (~1×106 cells/tube) were stained with DAPI (cell viability), anti-CD45-Pacific Blue, anti-F4/80-FITC, anti-CD11b-PercP Cy5.5, anti-Ly6G-APC, anti-CXCR2-PE (all R&D Systems), anti-CD62L-FITC, anti-GRK2-FITC, anti-Cytokeratin (C-11)-FITC (Abcam), anti-IL-33-PE (R&D Systems) and isotype controls (BD Biosciences), following the manufacturer’s protocols. IL-33 production by CD45− Cytokeratin+ epT cells was determined after stimulation with PMA (50 ng/ml) and ionomycin (1 μg/ml) in the presence of GolgiStop™ and permeabilization of the cells (Cytofix/Cytoperm, BD Biosciences). Cells were acquired using a Beckman Coulter CyAn™ ADP (Beckman Coulter, USA). Gating strategy (Supplementary Figure S5 online ) and analysis were performed using the FlowJo® software (treeStar Software, USA).
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