Magnetom tim trio mri

Manufactured by Siemens

Sourced in Germany

The Magnetom Tim Trio MRI is a magnetic resonance imaging (MRI) system developed by Siemens. It is designed to produce high-quality images of the human body for diagnostic purposes. The system utilizes a powerful superconducting magnet and advanced radio frequency (RF) technology to generate detailed images of the internal structures of the body.

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4 protocols using magnetom tim trio mri

Structural and Functional Brain Imaging

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All imaging data acquisition was performed at the National Yang Ming Chiao Tung University using a 3T Siemens Magnetom Tim Trio MRI scanner (Erlangen, Germany) with a 32-channel head coil. First, high-resolution T1-weighted MRI images were acquired with a three-dimensional magnetization-prepared rapid acquisition with gradient echo (3D MPRAGE) sequence (repetition time, TR: 3500 ms; echo time, TE: 3.5 ms; inversion time, TI: 1100 ms; field of view, FoV: 256 mm × 256 mm; 192 sagittal slices; flip angle, FA: 7 degrees; voxel size = 1 mm × 1 mm × 1 mm).
During the rsfMRI session, participants were instructed to stay awake, relax, and stare at the fixation point on the screen. In this session, T2-weighted images with blood oxygen level-dependent (BOLD) contrast were measured using a gradient echo-planar imaging (EPI) sequence (200 measurements; TR: 2500 ms; TE: 27 ms; FA: 77 degrees; FoV: 220 mm × 220 mm; matrix size = 64 × 64; voxel size = 3.4 mm × 3.4 mm × 3.4 mm).

Lin Y.C., Lien Y.R., Lin S.H., Kung Y.C., Huang C.C., Lin C.P, & Chang L.H. (2022). Baseline Cerebro-Cerebellar Functional Connectivity in Afferent and Efferent Pathways Reveal Dissociable Improvements in Visuomotor Learning. Frontiers in Neuroscience, 16, 904564.

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Functional MRI Protocol for Saccadic Eye Movement

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A 3 T Siemens Magnetom TIM Trio MRI scanner at the York MRI Facility was used to acquire fMRI data. An echo-planar imaging (EPI) sequence (repetition time [TR] = 2000 ms; echo time [TE] = 30 ms; flip angle [FA] = 90°; field of view [FOV] = 240 × 240 mm, matrix size = 80 × 80, in-plane resolution = 3 mm × 3 mm; slice thickness = 3 mm) was acquired in ascending, interleaved order for each of the six functional runs and for the two separate saccade localizer runs. Thirty-three contiguous slices were acquired per volume for a total of 192 volumes of functional data in each experimental run, and 128 volumes of data for each saccade control run. A T1-weighted anatomical reference volume was obtained for each participant using an MPRAGE sequence (TR = 1900 ms; FA = 256 mm × 256 mm; voxel size = 1 × 1 × 1 mm³). 192 slices were acquired per volume of anatomical data.

Baltaretu B.R., Dunkley B.T., Stevens W.D, & Crawford J.D. (2021). Occipital cortex is modulated by transsaccadic changes in spatial frequency: an fMRI study. Scientific Reports, 11, 8611.

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Aptamer-Mediated MRI Detection of PCa Cells

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To evaluate the ability of aptamer-conjugated GoldMag to target EpCAM-positive PCa cells for MRI in vitro, aptamer Eppc6 that has higher Kd compared with Eppc14 was primarily selected for detection. Three types of PCa cells PC-3, DU145 and LNCaP were, respectively, incubated with Eppc6-GoldMag at 37°C for 30 min. Subsequently, the cells were rinsed with washing buffer three times and resuspended in 3% agarose gel in eppendorf tubes, respectively. After the agarose solidification, the cells in eppendorf tubes were put in the test-tube stand and scanned by using a 3.0T MRI scanner (Siemens Magnetom Tim Trio MRI). The T2-weighted imaging (T2WI) of three types of PCa cell groups were, respectively, acquired with a repetition time (TR) of 3500 ms, an echo time (TE) of 90 ms, a field-of-view (FOV) of 109 mm, a slice thickness of 2.0 mm, and an average of 8. ssDNA-GoldMag served as a negative control. After MRI examination, the data were sent to a dedicated MRI image processing workstation (Leonardo; Siemens) for further analysis. Region of interest (ROI) was drawn in the longitudinal section of respective tubes to measure the T2WI signal intensity of PCa cells. At least three ROIs were drawn in each tube.

Zhong J., Ding J., Deng L., Xiang Y., Liu D., Zhang Y., Chen X, & Yang Q. (2021). Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer by Cell-SELEX for in vitro and in vivo MR Imaging. Drug Design, Development and Therapy, 15, 3985-3996.

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Resting-State fMRI Acquisition Protocol

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MRIs were acquired using a 3T Magnetom Tim Trio MRI scanner system (Siemens Medical Systems, Erlangen, Germany) using a 12-channel radiofrequency head coil. High-resolution anatomical images were collected using a T1-weighted 3D MPRAGE sequence (TR = 2500 ms, TE = 4.77 ms, TI = 1100 ms, acquisition matrix = 256 × 256 × 192, sagittal FOV = 256 mm, flip angle = 7°, voxel size = 1.0 × 1.0 × 1.0 mm 3 ). Whole-brain functional images were acquired using a T2*-weighted echo-planar imaging (EPI) sequence sensitive to blood-oxygenationlevel dependent (BOLD) contrast (TR = 2000 ms, TE = 30 ms, image matrix = 72 × 72, FOV = 216 mm, flip angle = 80°, slice thickness = 2.0 mm, distance factor = 20%, voxel size 3.0 × 3.0 × 3.0 mm 3 , 36 axial slices). 150 image volumes aligned to AC-PC were acquired. Participants were instructed to keep their eyes closed and relax during this resting state measurement.

Lisofsky N., Riediger M., Gallinat J., Lindenberger U, & Kühn S. (2016). Hormonal contraceptive use is associated with neural and affective changes in healthy young women. NeuroImage, 134.

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