The sections were deparaffinized, hydrated and incubated in citrate buffer with heating for 16 h at 60°C. Sections for immunohistochemistry were treated with 3% H2O2 for 10 min to quench the endogenous peroxidase activity. The sections were washed three times for 5 min in phosphate‐buffered saline (PBS) and blocked in 5% goat serum at room temperature for 1 h. The sections were stained with the primary antibody anti‐a‐smooth muscle actin (α‐SMA) (1:100, A11111, ABclonal) for immunohistochemistry. The sections were incubated with primary antibodies against COL1A1 (1:100, ab254113, Abcam), COL3A1 (1:100, AF5457, Affinity), α‐SMA (1:100, A11111, ABclonal), MMP2 (1:100, ab92536, Abcam), IL‐1β (1:100, A16288, ABclonal), and COX‐2 (1:100, ab179800, Abcam) for immunofluorescence. Species‐matched secondary antibody was used. Then, DAB (DAB‐0031, MXB‐Bio, China) was used for immunohistochemistry, and DAPI (ab104139, Abcam) was used for immunofluorescence.
A11111
Manufactured by ABclonal
Sourced in China
A11111 is a laboratory equipment designed for general use in scientific research and analysis. It serves as a tool to perform specific tasks within a laboratory setting. The core function of A11111 is to provide a controlled and reliable platform for scientific experimentation and data collection.
Automatically generated - may contain errors
Lab products found in correlation
Ab129068, by Abcam (1 mentions)
Ab133303, by Abcam (1 mentions)
Ab19870, by Abcam (1 mentions)
Ab92552, by Abcam (1 mentions)
Ab110304, by Abcam (1 mentions)
Ab134175, by Abcam (1 mentions)
Ac026, by ABclonal (1 mentions)
Goat anti rabbit igg h l cross adsorbed secondary antibody alexa fluor 555 a21428, by Thermo Fisher Scientific (1 mentions)
Pdgf bb, by R&D Systems (1 mentions)
Ab16502, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Ab131088, by Abcam (1 mentions)
Ab179800, by Abcam (1 mentions)
Ab254113, by Abcam (1 mentions)
Ab92536, by Abcam (1 mentions)
Ab104139, by Abcam (1 mentions)
Af5457, by Affinity Biosciences (1 mentions)
2 protocols using a11111
1
Immunohistochemical Analysis of Tissue Markers
2
Molecular Mechanisms of Vascular Remodeling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies against α-smooth muscle actin (α-SMA, A11111) and β-Actin (AC026) were purchased from Abclonal (Wuhan, China). Antibodies against Ac-p65 (ab19870), NF-κB (ab16502), NOX1 (ab131088), NOX2 (ab129068), NOX4 (ab133303), proliferating cell nuclear antigen (PCNA, ab92552), cyclin D1 (ab134175), and SIRT1 (ab110304) were obtained from Abcam (Cambridge, UK). PDGF-BB was purchased from R&D (Minneapolis, USA). Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody-Alexa Fluor 647 (A21235) and Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody-Alexa Fluor 555 (A21428) were purchased from Thermo Fisher Scientific (MA, USA). (R)-TML104 was synthesized and provided by Dr. Xun Sun's laboratory at the School of Pharmacy (Fudan University, China). Atorvastatin (MB1021) and resveratrol (MB1199) were purchased from Meilun Bio (Dalian, China). BAY 11-7082 (S1523) and N-acetyl-L-cysteine (S0077) was purchased from Beyotime (Shanghai, China). 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Solarbio (Beijing, China).
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