Aspartate aminotransferase ast

Methanol, acetic acid, quercetin, and gallic acid were purchased from Merck (Darmstadt, Germany). Sodium carbonate (Na₂CO₃), aluminum chloride, potassium acetate, and Folin-Ciocalteu’s reagent were procured from Sigma Aldrich, Inc (St Louis, MO). Isobutyl alcohol, magnesium carbonate (MgCO₃), iron (III) chloride (FeCl₃), acetone, chloroform, sodium nitroprusside, Methanol, and ferric chloride were source from BDH Chemical Ltd (Poole, England). Water used was glass distilled. Creatinine (Lot No. 315822), aspartate aminotransferase (AST; Lot No. 295800), alanine aminotransferase (ALT; Lot No. 295830), bilirubin (Lot No. 311158), uric acid (UA; Lot No. 309349), total protein (Lot No. 261368), and albumin (Lot No. 302605) kits were sourced from RANDOX Laboratories Ltd, Crumlin, Co, Antrim, UK. Kenxin refrigerated centrifuge model KX3400C was used, while a Jenway UV-visible spectrophotometer (Model 6305; Jenway, Barlo World Scientific, Dunmow, UK) was used to measure the absorbance.

ELISA kits (Cayman Chemical, MI, USA) for the determination of liver malondialdehyde (MDA) and superoxide dismutase (SOD) were done according to the written instructions. Serum levels of high sensitivity C-reactive protein (hs-CRP, ASSAYPRO, Saint Charles, MO, USA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (Randox Laboratories, Antrim, UK) were measured as per the manufacturer’s instructions.