Zikv strain prvabc59

Manufactured by American Type Culture Collection

ZIKV strain PRVABC59 is a laboratory-grown Zika virus strain isolated from a human patient in Puerto Rico in 2015. It is a viral isolate that can be used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Amicon ultra 15 centrifugal filter, by Merck Group (1 mentions)

Spelling variants of this product

PRVABC59 (13 citations) PRVABC59-ZIKV (2 citations)

5 protocols using zikv strain prvabc59

Assessing ZIKV and Influenza Immunity in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female 6-8-week-old C57BL/6 mice and breeding pairs for Tcf7^GFP and Ifnar1^-/- mice were purchased from Jackson laboratory. Tcf7^GFP and Ifnar1^-/- mice were bred in house in institutional animal facility. ZIKV strain PRVABC59 (ATCC) was propagated in Vero cell monolayer, titrated and stored in aliquots at -80°C until used.
C57BL/6 and Tcf7^GFP mice were infected with ZIKV strain PRVABC59 (1×10⁷ PFU, i/p). On day 37 p.i, the mice were sacrificed, and spleen were harvested to assess memory like NK cell responses or to purify NK cell subsets for adoptive transfer. The Ifnar1^-/- mice were adoptively transferred with various NK populations and infected a day later with 1×10⁵ PFU ZIKV PRVABC59 strain, i/p and viremia measurements were made at day 3 and 5 p.i. C57BL/6 mice were transferred with NK cell subsets and infected intranasally with 10³ FFU influenza A virus/Puerto Rico/8/34 (H1N1)(PR8) (Charles River lab)[50 (link)] and mice were sacrificed 5 days later.

Kujur W., Murillo O., Adduri R.S., Vankayalapati R., Konduru N.V, & Mulik S. (2020). Memory like NK cells display stem cell like properties after Zika virus infection. PLoS Pathogens, 16(12), e1009132.

+ Open protocol

+ Expand

Characterization of Anti-Flavivirus Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse anti-flavivirus antibody (clone D1-4G2-4-15 (4G2), RRID: Ab00230-2.0) and anti-ZIKV E protein DIII (LR) ZV-67 (RRID: Ab00812-2.0) was purchased from absolute antibody Inc., UK. FITC-conjugated goat anti-mouse polyclonal antibody was obtained from Sigma Inc. (MO, USA). Fluorescence-labeled anti-human CD45, CD4, CD19, CD14 and CD11c antibodies were purchased from BD Biosciences (CA, USA). Vero cells (strain Vero 76, clone E6, RRID: CVCL_0603) were obtained from ATCC and grown in Dulbecco's Modified Eagle medium (DMEM) containing Glutamax and supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin-streptomycin (PS) (Invitrogen) and maintained at 37 °C in the presence of 5% CO₂. ZIKV (strain PRVABC59) was obtained from ATCC. The viral stocks were produced by infecting Vero cells in the laboratory using an MOI (Multiplicity of infection) of 0.01. The supernatants were collected at day 4–5, then clarified, filtered, aliquoted and stored at − 80 °C. These viral stocks were used for FRNT (focus reduction neutralization test) neutralization assays and mice challenge.

Yi G., Xu X., Abraham S., Petersen S., Guo H., Ortega N., Shankar P, & Manjunath N. (2017). A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice. EBioMedicine, 25, 87-94.

+ Open protocol

+ Expand

Immunofluorescence Imaging of ZIKV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

ERmoxGFP was a gift from Dr. Erik Snapp (Albert Einstein College of Medicine, presently at Howard Hughes Medical Institute, Janelia Research Campus, Virginia) (Addgene plasmid #68,072), and Sec61β-GFP from Dr. Gia Voeltz (University of Colorado, Boulder). Mouse anti-dsRNA antibody (Cat#: J2-1904) was purchased from Scions English and Scientific Consulting, goat anti-rabbit Alexa Fluor 532 from ThermoFisher Scientific (Cat#: A-11009), goat anti-mouse Alexa Fluor 568 from ThermoFisher Scientific (Cat#: A-11031), and goat serum from Thermo Fisher Scientific (Cat#: 16,210–064). Rabbit anti-NS2B (Cat#: GTX133308) and NS4B (Cat#: GTX133321) were kindly provided by Genetex (Cat#: GTX133321). 16% paraformaldehyde (Cat#: 15,710) and 25% glutaraldehyde (Cat#: 16,220) were from Electron Microscopy Sciences, USA. All other chemicals were obtained from Sigma. ZIKV strain (PRVABC59) was obtained from ATCC (Cat#: VR-1843). All virus manipulations were performed according to level 2 containment procedures (Dr. Jean; UBC B17-0024).

Long R.K., Moriarty K.P., Cardoen B., Gao G., Vogl A.W., Jean F., Hamarneh G, & Nabi I.R. (2020). Super resolution microscopy and deep learning identify Zika virus reorganization of the endoplasmic reticulum. Scientific Reports, 10, 20937.

+ Open protocol

+ Expand

Zika Virus Stock Generation in C6/36 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

ZIKV stocks were generated in Aedes albopictus clone C6/36 cells as previously described [40] (link). The ZIKV PRVABC59 strain was purchased from American Type Culture Collection (ATCC; Manassas, VA). The ZIKV MR766 stock was purchased from Zeptomatrix, Buffalo, NY. Briefly, a T-75 flask of C6/36 cells (90%–95% confluency) was inoculated with 1 × 10⁶ ZIKV virions in low volume (3 ml) for 1 h, rocking it every 15 min. After 1 h, 17 ml of media was added and C6/36 cells were maintained at 28 °C in 5% CO₂. At 7–8 days post-viral inoculation, supernatants were harvested, filtered, and stored at −80 °C. ZIKV titer was determined by an FFU assay.

Song G., Lee E.M., Pan J., Xu M., Rho H.S., Cheng Y., Whitt N., Yang S., Kouznetsova J., Klumpp-Thomas C., Michael S.G., Moore C., Yoon K.J., Christian K.M., Simeonov A., Huang W., Xia M., Huang R., Lal-Nag M., Tang H., Zheng W., Qian J., Song H., Ming G.L, & Zhu H. (2021). An Integrated Systems Biology Approach Identifies the Proteasome as A Critical Host Machinery for ZIKV and DENV Replication. Genomics, Proteomics & Bioinformatics, 19(1), 108-122.

+ Open protocol

+ Expand

Detailed Preparation of Dengue and Zika Virus Stocks

Check if the same lab product or an alternative is used in the 5 most similar protocols

DENV1 (Hawaii strain), DENV2 (NGC strain), and DENV3 (H87 strain) were provided by the Center of Disease Control, Department of Health, Taiwan and prepared from supernatants of infected C6/36 cells. ZIKV (PRVABC59 strain) was obtained from the American Type Culture Collection (ATCC) and amplified in Vero cells. The infectious cDNA plasmids of DENV4 containing full-length cDNA copies of DENV4 strain 814669, its 3' noncoding region deletion vaccine candidate strain DENV4Δ30, and DENV2/4Δ30 containing the prM and E genes of the DENV2 NGC strain were obtained from the US NIH. Culture supernatants were collected at 8 days post-transfection. To prepare high DENV titers, viruses were concentrated using a 10-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). All virus stocks were stored at -80^oC until further used.

Lin H.H., Yang S.P., Tsai M.J., Lin G.C., Wu H.C, & Wu S.C. (2019). Dengue and Zika Virus Domain III-Flagellin Fusion and Glycan-Masking E Antigen for Prime-Boost Immunization. Theranostics, 9(16), 4811-4826.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!