Mice were administered ketamine and xylazine (100 mg/kg and 10 mg/kg, respectively) and euthanized by transcardial perfusion with 10 mL of PBS, followed by 10 mL of 4% paraformaldehyde in PBS. Brains were extracted and 100 μm coronal sections were cut on a vibratome. The tissue was labeled with the following antibodies: goat anti-GFP (Abcam ab6673), rabbit anti-arc (Synaptic Systems 156-003), goat anti-c-fos (Santa Cruz sc-52-G), alexa-488 donkey anti-goat (Jackson ImmunoResearch), alexa-568 donkey anti-rabbit (Life Technologies A11057), alexa-647 donkey anti-rabbit (Jackson ImmunoResearch 711-605-152) and alexa-568 donkey anti-goat (Life Technologies A10042). Slices were counterstained with neurotrace 640/660 or 435/455 (Life Technologies N21483 or N21479, respectively). Antibody amplification was not used to visualize eNpHR3.0-eYFP. All images were taken using a Zeiss LSM-710 confocal microscope system.
Ab6673
Manufactured by Synaptic Systems
Ab6673 is a laboratory equipment product. It serves a core function within the research and scientific community, but a detailed description cannot be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
Donkey anti goat alexa568, by Thermo Fisher Scientific (2 mentions)
Alexa 647 donkey anti rabbit, by Jackson ImmunoResearch (2 mentions)
Alexa 488 donkey anti goat, by Jackson ImmunoResearch (2 mentions)
Neurotrace 640 660, by Thermo Fisher Scientific (2 mentions)
Lsm 710 confocal microscope system, by Zeiss (2 mentions)
Donkey anti rabbit alexa 568, by Thermo Fisher Scientific (2 mentions)
Goat anti c fos, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti arc, by Synaptic Systems (2 mentions)
2 protocols using ab6673
1
Immunostaining of Mouse Brain Sections
2
Immunostaining of Mouse Brain Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were administered ketamine and xylazine (100 mg/kg and 10 mg/kg, respectively) and euthanized by transcardial perfusion with 10 mL of PBS, followed by 10 mL of 4% paraformaldehyde in PBS. Brains were extracted and 100 μm coronal sections were cut on a vibratome. The tissue was labeled with the following antibodies: goat anti-GFP (Abcam ab6673), rabbit anti-arc (Synaptic Systems 156-003), goat anti-c-fos (Santa Cruz sc-52-G), alexa-488 donkey anti-goat (Jackson ImmunoResearch), alexa-568 donkey anti-rabbit (Life Technologies A11057), alexa-647 donkey anti-rabbit (Jackson ImmunoResearch 711-605-152) and alexa-568 donkey anti-goat (Life Technologies A10042). Slices were counterstained with neurotrace 640/660 or 435/455 (Life Technologies N21483 or N21479, respectively). Antibody amplification was not used to visualize eNpHR3.0-eYFP. All images were taken using a Zeiss LSM-710 confocal microscope system.
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