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4 protocols using peg 200

1

Solubility Enhancement of Drugs

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EA was purchased from Yucca Enterprises Pvt. Ltd, Mumbai, India. Capmul MCM C8, and captex 500 were gifted by Abitec Corp., USA. Capryol 90, labrafil M 2125 CS, labrafil M 2130 CS, labrafac CC, labrasol, lauroglycol FCC, transcutol P were kindly gifted by Gattefosse, France. Oleic acid, tween 80, tween 20, span 20, propylene glycol, PEG 400 and PEG 200 were purchased from Loba Chemie Pvt. Ltd., Mumbai. Cremophor RH 40 was a gift from BASF, Germany.
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2

Wet Salted Sheepskin Dehairing Protocol

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Wet salted red hair sheepskins were chosen as raw material for the experiments and were procured from a slaughterhouse located at Chennai, India. Commercial grade dehairing enzyme, (Microdep C, protease formulation) was procured from Tex Biosciences Pvt. Ltd. India and commercial grade alpha-amylase (fiber opening enzyme) was procured from United Alacrity India Pvt Ltd., India. All chemicals used for leather processing were of commercial grade. Solvents namely ethanol, ethyl acetate, ethyl lactate, heptane, propylene carbonate, propylene glycol and PEG 200 were of analytical grade and purchased from Loba Chemie, India. All the reagents used for the assay were of analytical grade and purchased from SRL Pvt. Ltd., India.
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3

Characterization and Antibacterial Activity of ZnO NPs

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Zinc oxide NPs dispersion purchased from
Sigma-Aldrich (catalog no. 721077) with reported particle size less
than 100 nm (measured by DLS) and an average particle size less than
40 nm (Aerodynamic Particle Seizer, APS) was used in this study. PEG-200
and PEG-6000 used for the surface modification of ZnO NPs were procured
from Loba Chemie (CAS no. 25322-68-3) and S-D fine-Chem Limited (product
code 3957P25; CASR no. 25322-68-3), respectively. PD solution IP (DIANEAL
PD-2 with 2.5% w/v dextrose) was obtained from Baxter Healthcare.
The sodium salt of trimethylsilylpropionic acid-d4 (TSP)
and deuterium oxide (D2O) used in NMR spectroscopy were
procured from Sigma-Aldrich (Rhode Island, USA). The deionized water
was used throughout the experiments (obtained from Millipore system
equipped with Milli-Q Gradient Century and Elix Century systems).
Gram-positive S. aureus (MTCC-3160)
and Gram-negative E. coli (DH5-Alpha)
strains were used in antibacterial assessment of ZnO NPs. LB media
purchased from Himedia (REF M575-500G) was used for the bacterial
strain growth.
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4

Optimizing Topical Capsaicin Formulations

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Capsaicin was provided as a gift sample by Naturite Agro Products Limited Hyderabad, India. Labrafil M1944CS, Caproyl PGMC, Labrasol was obtained from Gattefosse India Pvt. Ltd, France as a kind gift sample. Span 80, Span 20, Tween 20, isopropyl alcohol, polyethylene glycol (PEG) 200, PEG 400, PEG 600, ethyl oleate, Labrafil M1944CS, labrafac PG, castor oil, oleic acid, isopropyl myristate (IPM), cotton seed oil, soybean oil, & olive oil were procured from LobaChemie Pvt. Ltd, Mumbai, India. Cremophor EL & Cremophor RH40 was provided by BASF, Germany. All other chemicals used were of analytical grade.
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