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Enolase 1

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Enolase-1 is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis. It is a key component in the metabolic pathway that converts glucose to energy.

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Lab products found in correlation

Protease phosphatase inhibitor cocktail, by Roche (2 mentions) Hif 1α antibody, by BD (2 mentions) Phospho alky1604, by Cell Signaling Technology (2 mentions) Antibodies for glut1 and glut3, by Abcam (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) β tubulin, by Cell Signaling Technology (2 mentions) Phospho akt s473, by Cell Signaling Technology (2 mentions) Phospho 4ebp1 t37 46, by Cell Signaling Technology (2 mentions) Pgam1, by Cell Signaling Technology (2 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) Tyro3, by Cell Signaling Technology (1 mentions) Sc 2020, by Santa Cruz Biotechnology (1 mentions) Stat6, by Cell Signaling Technology (1 mentions) P stat6, by Cell Signaling Technology (1 mentions) Cd11b apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd25 alexafluor488, by Thermo Fisher Scientific (1 mentions) Bip grp78, by Cell Signaling Technology (1 mentions)

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Anti-enolase 1 (6 citations)

4 protocols using enolase 1

1

Western Blotting Antibody Panel

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For western blotting, the following antibodies were obtained from Cell Signaling: pAKT (S473, Cat# 9271), AKT (Cat# 9272), Enolase-1 (Cat# 3810), pERK1/2 (T202/Y204, Cat# 9106), ERK1/2 (Cat# 9102), PARP (Cat# 9542), pSTAT6 (Y641, Cat# 9361), STAT6 (Cat# 9362), TYRO3 (Cat# 5585), and α-tubulin (Cat# 2125). Additional antibodies used for western blotting include AXL (R&D Systems, AF154), pMER (Y749, Y753, Y754, PhosphoSolutions), and MER (Abcam 52968). Horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit, Bio-Rad 170-6515; goat anti-mouse, Bio-Rad 170-6516; donkey anti-goat, Santa Cruz sc-2020) were used for enhanced chemiluminescence of western blots. The following antibodies were used for measurement of indirect immunofluorescence using flow cytometry: mouse monoclonal anti-MER (Caveo Therapeutics, CVO-590) and phycoerythrin-conjugated donkey anti-mouse (Jackson Immunoresearch, 715-116-150) (Figure 1) or Mer590 and allophycocyanin-conjugated donkey anti-mouse (Jackson Immunoresearch, 715-136-150) (Figure 2). All antibodies were used as recommended by the manufacturer unless otherwise specified.
Cummings C.T., Linger R.M., Cohen R.A., Sather S., Kirkpatrick G.D., Davies K.D., DeRyckere D., Earp H.S, & Graham D.K. (2014). Mer590, a novel monoclonal antibody targeting MER receptor tyrosine kinase, decreases colony formation and increases chemosensitivity in non-small cell lung cancer. Oncotarget, 5(21), 10434-10445.
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2

Immunoblotting Analysis of Metabolic Regulators

Cited 1 time
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Lysates from cells or xenografts were prepared in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). Proteins were analyzed by immunoblotting as previously described.58 (link) The antibodies for ALK, β-tubulin, phospho-ALKY1604, HK1, HK2, PFK1, GAPDH, PKM2, LDHA, PGAM1, Enolase-1, AKT, phospho-AKTS473 and phospho-4E-BP1T37/46 were purchased from Cell Signaling. The antibodies for GLUT1 and GLUT3 were obtained from Abcam (Cambridge, MA). The HIF1α antibody was from BD Biosciences (San Jose, CA).
Ma Y., Yu C., Mohamed E.M., Shao H., Wang L., Sundaresan G., Zweit J., Idowu M, & Fang X. (2016). A causal link from ALK to hexokinase II overexpression and hyperactive glycolysis in EML4-ALK-positive lung cancer. Oncogene, 35(47), 6132-6142.
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3

Immunoblotting Analysis of Metabolic Regulators

Cited 1 time
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Lysates from cells or xenografts were prepared in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). Proteins were analyzed by immunoblotting as previously described.58 (link) The antibodies for ALK, β-tubulin, phospho-ALKY1604, HK1, HK2, PFK1, GAPDH, PKM2, LDHA, PGAM1, Enolase-1, AKT, phospho-AKTS473 and phospho-4E-BP1T37/46 were purchased from Cell Signaling. The antibodies for GLUT1 and GLUT3 were obtained from Abcam (Cambridge, MA). The HIF1α antibody was from BD Biosciences (San Jose, CA).
Ma Y., Yu C., Mohamed E.M., Shao H., Wang L., Sundaresan G., Zweit J., Idowu M, & Fang X. (2016). A causal link from ALK to hexokinase II overexpression and hyperactive glycolysis in EML4-ALK-positive lung cancer. Oncogene, 35(47), 6132-6142.
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4

Antibodies for Western Blotting and Flow Cytometry

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Antibodies for Western blotting were purchased from the following sources: Histone H2A (Cell Signaling Technology #3636); HMGB1 (Cell Signaling Technology #6893); β-Actin (Cell Signaling Technology #3700); Profilin-1 (Cell Signaling Technology #3237); Enolase-1 (Cell Signaling Technology #3810); Bip/GRP78 (Cell Signaling Technology #3177); Alpha-fodrin (EMD Millipore #MAB1622); MLKL (Cell Signaling Technology #14993); Phospho-MLKL Ser358 (Cell Signaling Technology #91689); GAPDH (ThermoFisher #AM4300). Antibodies for flow cytometry were purchased from the following sources: F4/80, PE-Cyanine5 (ThermoFisher #15-4801-82); CD11b APC-eFluor 780 (ThermoFisher #47-0112-82); CD11c Alexa Fluor 700 (ThermoFisher #56-0114-82); CD86 (B7-2) FITC (ThermoFisher #11-0862-82); CD40 Super Bright 436 (ThermoFisher #62-0401-82); MHC Class II I-Ab (ThermoFisher #17-5320-82); CD25 Alexa Fluor 488 (ThermoFisher #53-0251-82); CD69 Alexa Fluor 700 (BioLegend #104539).
Andersohn A., Garcia M.I., Fan Y., Thompson M.C., Akimzhanov A.M., Abdullahi A., Jeschke M.G, & Boehning D. (2019). Aggregated and Hyperstable Damage-Associated Molecular Patterns Are Released During ER Stress to Modulate Immune Function. Frontiers in Cell and Developmental Biology, 7, 198.
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