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Elisa kit for klotho

Manufactured by MyBioSource
Sourced in United States

The ELISA kit for Klotho is a laboratory tool used to detect and quantify the Klotho protein in biological samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the levels of Klotho in a reliable and reproducible manner.

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2 protocols using elisa kit for klotho

1

Klotho Quantification in Porcine Follicular Fluid

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Porcine follicular fluid (PFF) from 2-5 mm- and 5-8 mm-sized follicles was collected from porcine ovaries, and used to determine the concentration of KP. ELISA kit for Klotho (MBS9359499, Mybiosource, San Diego, CA, USA) was used and KP concentration was measured according to the manufacturer’s instruction. The concentration was expressed as pg per ml of PFF.
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2

Klotho and Inflammation Signaling

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Antibodies against ICAM-1, VCAM-1 and GAPDH were purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). Antibody against Klotho was purchased from Abcam Inc. (Cambridge, MA). Recombinant Klotho protein, recombinant IL-37 protein, and Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 were purchased from the R&D System (Minneapolis, MN). ELISA kit for Klotho was purchased from MyBioSource (San Diego, CA). Lipopolysaccharide (LPS, Escherichia coli 0111:B4) and all other chemicals and reagents were purchased from Sigma-Aldrich Chemical (St Louis, MO).
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