Qfikit

Manufactured by Agilent Technologies

The QFIKIT is a compact and portable device designed for quantitative flow injection analysis. It provides accurate and reliable measurements of various analytes in liquid samples. The QFIKIT operates on the principle of flow injection analysis, allowing for automated sample handling and measurement processing. Its core function is to facilitate efficient and precise quantitative analysis of target substances in a range of applications.

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Lab products found in correlation

Mab3582, by R&D Systems (1 mentions) Guava easycyte ht cytometer, by Merck Group (1 mentions)

2 protocols using qfikit

Quantifying Cell Surface Receptor Levels

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Example 9

Receptor surface expression levels on selected cell lines were determined using the QFIKIT (Dako K0078) employing flow cytometry, the results of which are shown in FIG. 18. Briefly, five populations of calibration beads presenting different numbers of mouse mAb molecules on their surfaces were used as a calibration standard. 1.5×105 cells/well were labeled with primary mouse anti-EGFR (ab187287, Abcam) and mouse anti-c-MET antibodies (MAB3582, R&D Systems) at saturating doses (5 μg/ml). Then, beads and cells were stained with secondary goat anti-mouse Fc F(ab′)2 FITC conjugate (10 μg/ml, Jackson Immuno Research) and were subjected to flow cytometry measurement using a Guava easyCyte HT cytometer (Millipore). Beads and cells were measured on the same day using the same settings. Based on a calibration line for fluorescence of beads versus bead surface density, antigen cell surface densities for c-MET and EGFR were calculated.

US11235063B2. Bi-specific antibodies for enhanced tumor selectivity and inhibition and uses thereof (2022-02-01). MERCK PATENT GMBH [DE]. Inventors: Achim Doerner [DE], Lars Toleikis [DE], Vanita D. Sood [US], Carolin Sellmann [DE], Christine Knuehl [DE].

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Quantifying CD47 and CD19 Expression

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CD47 and CD19 expression was determined using QFIKIT (Dako K0078) containing five different populations of beads bearing different numbers of mouse mAb. Cells were labelled with a saturating dose (10 μg ml⁻¹) of primary mouse mAb against the antigen of interest (anti-CD19, R&Dsystems MAB4867; anti-CD47, eBiosciences B6H12). Beads and cells were then stained with an anti-mouse Fc-FITC (dilution 1:50; included in the Dako kit) and analysed by flow cytometry. Cell fluorescence is then compared with the bead standards to estimate antigen quantity on the surface of cells.

Fischer N., Elson G., Magistrelli G., Dheilly E., Fouque N., Laurendon A., Gueneau F., Ravn U., Depoisier J.F., Moine V., Raimondi S., Malinge P., Di Grazia L., Rousseau F., Poitevin Y., Calloud S., Cayatte P.A., Alcoz M., Pontini G., Fagète S., Broyer L., Corbier M., Schrag D., Didelot G., Bosson N., Costes N., Cons L., Buatois V., Johnson Z., Ferlin W., Masternak K, & Kosco-Vilbois M. (2015). Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nature Communications, 6, 6113.

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