Bromelain, trypsin, papain, 2,2'-diphenyl-1-picrylhydrazyl,2,2′-Azino-bis(3ethylbenzothiazoline-6-sulfonic acid, α-Glucosidase, α-amylase, N-hydrate Hipuril-His-Leu, angiotensin-converting enzyme rabbit were from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA) and neutral protease were from Novozymes (Denmark).
Neutral protease
Manufactured by Novozymes
Sourced in China
Neutral protease is an enzyme that hydrolyzes peptide bonds in proteins. It is effective in a neutral pH range and can be used to break down proteins in various industrial and biotechnological applications.
Automatically generated - may contain errors
Lab products found in correlation
Papain, by Novozymes (2 mentions)
Trypsin, by Merck Group (1 mentions)
Papain, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
Bromelain, by Merck Group (1 mentions)
Cck 8, by MedChemExpress (1 mentions)
Biochemical kit, by Nanjing Jiancheng (1 mentions)
Alcalase, by Novozymes (1 mentions)
Trypsin, by Novozymes (1 mentions)
Sodium cholate, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
α amylase, by Novozymes (1 mentions)
Thermostable α amylase, by Novozymes (1 mentions)
Neutrase 0.8 l, by Novozymes (1 mentions)
6 protocols using neutral protease
1
Enzyme Activity Assay Protocol
2
Soy Meal Peptide Synthesis and Evaluation
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Soy meal (Shandong Yuwang Industrial Co. LTD, China), PP (KYKRQRW) was synthesized by Qiangyao Biological Technology Co., Ltd. Alcalase, papain, and neutral protease were obtained from Novozymes Biotechnology Co., LTD. Caco-2 cells (TCHu146) were purchased from Cells bank, Shanghai Chinese Academy of Sciences, CCK8 was obtained from MedChemExpress, Shanghai. All chemical reagents were analytically pure. Kunming male mice (KM) were obtained from the Animal Experiment Center of Guangdong University of Chinese Medicine. Zinc deficiency feed was purchased from Nantong Tuofei Feed Technology Co. LTD. Biochemical kits were purchased from Nanjing Jiancheng Biotechnology Co. LTD, China.
3
Fish Scale Proteases Extraction
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Ctenopharyngodon idella scales were purchased from a local supermarket (Wuhan, China), washed, dried, and stored at room temperature until use. Novozymes (Beijing, China) Investment Co., Ltd. was the source of the alcalase, papain, trypsin, neutral protease, and pepsin protease and bovine serum albumin (BSA). Sephadex G-50 and ion exchange (sulfopropyl-Sephadex C-25) were provided by Yuanye Chemical Scientific Co. (Shanghai, China). All chemicals employed were reagent grade or higher.
4
Pineapple Bioactive Compound Extraction
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Ripened pineapple fruits were purchased from a commercial orchards located in Xuwen country (Guangdong, China). α-Amylase, neutral protease and cellulase R-10 were bought in Novozymes Biotechnology Co., Ltd. (Copenhagen, Danish). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), glucose, cholesterol and sodium cholate were purchased from Sigma Aldrich Chemical Co. (Milwaukee, WI, USA). All other reagents were of analytical grade.
5
Rice Bran Processing and Enzyme Extraction
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Rice bran was obtained from Hunan Hekang Ecological Agriculture Development Co., Ltd. (Yiyang, China), milled through a 40-mesh sieve, sealed in polyethylene bags, and kept at room temperature for further analysis. Thermostable α-amylase (EC 3.2.1.1, Termamyl DS SC, from Bacillus amyloliquefaciens) and neutral protease (EC 3.4.21.62, Neutrase 0.8 L, from Bacillus amyloliquefaciens) were supplied by Novozymes (Beijing, China). Amberlite XAD-2 was obtained from Rohm and Haas (Philadelphia, PA, USA). Xylanase (EC 3.2.1.8, from Trichoderma viride), ferulic acid, arabinose, and xylose were purchased from Aladdin Industrial Inc. (Shanghai, China). All other chemicals and solvents used were of analytical grade or above.
6
Corn and Cassava Starch Fermentation
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Corn (Xiannong Seed Co., LTD, Nanchang, China); the content of starch, protein and fat was 71.52%, 8.50% and 3.88%, respectively; the bulk density and relative density was 778.71% and 1.29%, respectively) and cassava starch (Hengrui Technology, Luohe, China) were obtained from a local supermarket. Neutral protease (1.27 × 105 U/mL) was obtained from Novozymes (Copenhagen, Denmark). Xanthan gum and guar gum was obtained from Danisco (Wilmington, DE, USA).
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