Kieselgel 60 f254 320 400 mesh

Anhydrous solvents and reagents were purchased from Sigma Aldrich (Gillingham, UK) and were used without additional purification. N-methyl-N-(2-hydroxyethyl)-4-aminobenzaldehyde was purchased from abcr GmbH (Karlsruhe, Germany). Flash column chromatography purification was performed on silica gel (Merck Kieselgel 60 F₂₅₄ 320–400 mesh). Thin Layer Chromatography (TLC) was performed on Merck aluminium-backed plates pre-coated with silica (0.2 mm, 60 F₂₅₄) which were visualised by quenching of ultraviolet fluorescence (λ = 254 and 365 nm). ¹H-NMR, ¹³C-NMR and ¹⁹F-NMR was obtained using a Bruker AV-400 spectrometer at a frequency of 400, 101 and 376 MHz, respectively. Chemical shifts (δ) are given in parts per million (ppm) and referenced to the appropriate residual solvent peaks. Signals are assigned as s, d, t, dt, m and br for singlet, doublet, triplet, double triplet, multiplet and broad respectively. Mass spectrometry was performed by the Mass Spectrometry Facility of the Chemistry Department of Imperial College London. Excitation (λ_ex) and emission (λ_em) spectra were obtained using an Infinite 200 PRO plate reader (Tecan, Männedorf, Switzerland).

Anhydrous solvents and reagents were purchased from Sigma Aldrich (Gillingham, UK) and were used without additional purification. Flash column chromatography purification was performed on silica gel (Merck Kieselgel 60 F₂₅₄ 320–400 mesh). Thin Layer Chromatography (TLC) was performed on Merck aluminium-backed plates pre-coated with silica (0.2 mm, 60 F₂₅₄) which were visualised by quenching of ultraviolet fluorescence (λ = 254 and 365 nm). ¹H-NMR, ¹³C-NMR and ¹⁹F-NMR was obtained using a Bruker AV-400 spectrometer at a frequency of 400, 101 and 376 MHz, respectively. Chemical shifts (δ) are given in parts per million (ppm) and referenced to the appropriate residual solvent peaks. Signals are assigned as s, d, t, dt, m and br for singlet, doublet, triplet, double triplet, multiplet and broad respectively. Mass spectrometry was performed by the Mass Spectrometry Facility of the Chemistry Department of Imperial College London. Determination of log D_7.5 was performed by the shake-flask method following a published procedure.^{20 (link)}