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Bicr 18

Manufactured by Merck Group

The BICR 18 is a laboratory instrument designed for conducting various analytical and research procedures. It is a versatile piece of equipment that can be used in a range of scientific applications. The core function of the BICR 18 is to provide a controlled and consistent environment for conducting experiments and analyses. The specific details of its intended use and capabilities are not available in this factual and unbiased description.

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2 protocols using bicr 18

1

Cell Line Verification and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cal27 and Detroit 562 cells were obtained from ATCC (Manassas, VA). 686LN cells were obtained from Georgia Chen at MD Anderson Cancer Center (Houston, TX). BICR 18 and PE/CA-PJ49 cells were obtained from Sigma-Aldrich (St. Louis, MO). UMSCC cell lines were obtained from Thomas E. Cary at the University of Michigan (Ann Arbor, MI). HSC-2 cells were obtained from Hideo Niwa at Nihon University (Tokyo, Japan). Cal27, Detroit 562, HSC-2, UMSCC 47, and UMSCC 22A were maintained in DMEM (Corning, Corning, NY) containing 10% fetal bovine serum (FBS; Gemini Bio-Products, West Sacramento, CA). UMSCC 1 were maintained in DMEM containing 10% FBS and 0.4 μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO). BICR 18 were maintained DMEM containing 10% FBS, 0.4 μg/mL hydrocortisone, and 2 mM L-glutamine (Life Technologies, Carlsbad, CA). 686LN were maintained in DMEM/F12 (Life Technologies, Carlsbad, CA) containing 10% FBS. PE/CA-PJ49 were maintained in Iscove’s DMEM (Corning, Corning, NY) containing 10% FBS and 2 mM L-glutamine. All cells were genotypically verified using the AmpFSTR Identifiler PCR Amplification Kit according to the manufacturer’s instructions (Life Technologies, Carlsbad, CA) and maintained at 37°C and 5% CO2.
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2

Cell Line Verification and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cal27 and Detroit 562 cells were obtained from ATCC (Manassas, VA). 686LN cells were obtained from Georgia Chen at MD Anderson Cancer Center (Houston, TX). BICR 18 and PE/CA-PJ49 cells were obtained from Sigma-Aldrich (St. Louis, MO). UMSCC cell lines were obtained from Thomas E. Cary at the University of Michigan (Ann Arbor, MI). HSC-2 cells were obtained from Hideo Niwa at Nihon University (Tokyo, Japan). Cal27, Detroit 562, HSC-2, UMSCC 47, and UMSCC 22A were maintained in DMEM (Corning, Corning, NY) containing 10% fetal bovine serum (FBS; Gemini Bio-Products, West Sacramento, CA). UMSCC 1 were maintained in DMEM containing 10% FBS and 0.4 μg/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO). BICR 18 were maintained DMEM containing 10% FBS, 0.4 μg/mL hydrocortisone, and 2 mM L-glutamine (Life Technologies, Carlsbad, CA). 686LN were maintained in DMEM/F12 (Life Technologies, Carlsbad, CA) containing 10% FBS. PE/CA-PJ49 were maintained in Iscove’s DMEM (Corning, Corning, NY) containing 10% FBS and 2 mM L-glutamine. All cells were genotypically verified using the AmpFSTR Identifiler PCR Amplification Kit according to the manufacturer’s instructions (Life Technologies, Carlsbad, CA) and maintained at 37°C and 5% CO2.
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