Cholestane

Manufactured by Merck Group

Sourced in United States

Cholestane is a chemical compound that is commonly used as a standard reference material in analytical chemistry. It is a saturated steroid hydrocarbon with the chemical formula C27H48. Cholestane is often used in the analysis and identification of steroids and other lipid compounds.

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Close spelling variants of this product

5α-cholestane Cholestane spin-label (CSL) 5‐alpha‐cholestane

3 protocols using cholestane

Characterization of Egg Yolk Lipids

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Natural eggs were purchased from the local market, and the yolk was separated from the albumen manually. Powdered egg yolk was supplied by the company Huevos Maryper (Murcia, Spain) and duck liver pâté by Malvasia, S.A (Soria, Spain).
β-CD (purity 99.5%) was purchased from Shandong Xinda Fine Chemical Co., Ltd. (Qingdao, China). Standards of fatty acid methyl esters, cholesterol, cholestane, and all reagent grades were supplied from Sigma (St. Louis, MO, USA).

Alonso L., Calvo M.V, & Fontecha J. (2019). The Influence of β-Cyclodextrin on the Reduction of Cholesterol Content in Egg and Duck Liver Pâté. Foods, 8(7), 241.

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Sterol Extraction and Characterization

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All reagents were analytical grade. Cyclohexane and potassium hydroxide were obtained from Fisher Scientific, Co. (Fair Lawn, NJ, USA). Absolute ethanol was obtained from Merck (Merck KGaA, Darmstadt, Germany). Campesterol, cholestane, cholesterol, stigmasterol, and sitosterol standards were obtained from Sigma-Aldrich, Inc. (Sigma Chemical, St. Louis, MO, USA). The trimethylsilyl (TMS) ether derivatives of all sterols were prepared using N,O-bis-[trimethylsilyl]trifluroacetamide reagent (Pierce Chemical Co., Rockford, IL, USA).

Hsu C.C., Kuo H.C, & Huang K.E. (2017). The Effects of Phytosterols Extracted from Diascorea alata on the Antioxidant Activity, Plasma Lipids, and Hematological Profiles in Taiwanese Menopausal Women. Nutrients, 9(12), 1320.

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Analytical Standards for Sterol Quantification

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desmosterol-d 6, zymosterol-d 5 , zymosterol and lanosterol-d 6 were obtained from Avanti lipids (Alabaster, AL, USA). Tert-butylhydroxytoluene (BHT), cholesterol, -cholestane, 7-hydroxycholesterol, 7-dehydrocholesterol and 7-ketocholesterol and squalene were from Sigma (St. Louis, MO, USA). Lathosterol, lanosterol, desmosterol, 27-hydroxycholesterol, campesterol, and were obtained from Steraloids (Newport, RI, USA). Campesterol-d 3, 7hydroxycholesterol-d 7 , Lathosterol-d 4 and 7-ketocholesterol-d 7 were purchased from CDN Isotopes (Quebec, Canada). 27-hydroxycholesterol-d 5 , 24-hydroxycholesterol and 24hydroxycholestero-d 7 were from Medical Isotopes , Inc (Pelham, AL, USA). squalene-d 6 and 24,25-dihydrolanosterol-d 6 was obtained from Toronto research chemicals (TRC, Ontario, Canada). All standards obtained were of the highest purity (>95%). Methanol, hexane, methyl tert-butyl ether (MTBE), acetonitrile, toluene, formic acid and NaOH were purchased from Ajax Finechem (Thermo Fisher Scientific, AU). CUQAX223 UCT Clean-Up QAX2 solid phase extraction columns and BSTFA (N,O-bis(trimethylsilyl) trifluoroacetamide) + 1% TMCS (trimethylchlorosilane) was purchased from PM Separations (Qld, Australia).

Kreilaus F., Spiro A.S., McLean C.A., Garner B, & Jenner A.M. (2016). Evidence for altered cholesterol metabolism in Huntington's disease post mortem brain tissue. Neuropathology and applied neurobiology, 42(6).

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