Anti human cd32 antibody clone 4 3

All reagents were purchased from Thermo Fischer Scientific (Waltham, MA, USA) unless otherwise stated. Chronolume, used for the detection of secreted ATP, was purchased from Chrono‐log Corporation (Havertown, PA, USA). The CLEC‐2 activating antibody for human was purchased from R & D Systems (Minneapolis, MN, USA). The CLEC‐2 activating antibody (clone 17D9) for mouse was purchased from BioLegend (San Diego, CA, USA) and the donkey anti‐rat (DAR) IgG was purchased from Novex (Wadsworth, OH, USA). Anti‐pSyk Y352 (Y346 in mouse) and anti‐pSyk Y348 (Y342 in mice) were purchased from Abcam (Cambridge, England). Anti‐Syk and anti‐PLCγ2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐pSyk Y525/526 (mouse Y519/520), anti‐pPLCγ2 Y1217, and anti‐pLAT Y191 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti‐LAT was purchased from EMD Millipore (Burlington, MA, USA). Anti‐human CD32 antibody, clone IV.3 was obtained from STEMCELL Technologies (Vancouver, BC, Canada). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti‐rabbit and IRDye 680LT goat anti‐mouse were purchased from Li‐Cor (Lincoln, NE, USA).