Hpa001239

Manufactured by Merck Group

Sourced in United Kingdom

HPA001239 is a laboratory equipment product from the Merck Group. It serves as a core tool for various scientific applications in research and development settings. The device's primary function is to facilitate precise measurements and data collection, enabling researchers to conduct their studies effectively. No further details or interpretations are provided.

Automatically generated - may contain errors

Lab products found in correlation

Pvdf membrane, by Bio-Rad (1 mentions) Ab209813, by Abcam (1 mentions) Ab156034, by Abcam (1 mentions) Ab8226, by Abcam (1 mentions) Ab205718, by Abcam (1 mentions) Imagej, by LI COR (1 mentions) Sc 271185, by Santa Cruz Biotechnology (1 mentions) A11534, by ABclonal (1 mentions)

2 protocols using hpa001239

Cardiac Patch Protein Expression Analysis

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The cardiac patches were cultured for a week. At day 7, the cell lysates were collected using RIPA buffer (ab156034, Abcam, United Kingdom) with protease inhibitor (Calbiochem, USA) and ran on 8% SDS-PAGE gel (Bio-Rad, USA). Then proteins were transferred to PVDF membrane (Bio-Rad, USA). Immunoblotting was performed using antibodies specific to TNNT2 (ab209813, Abcam, UK), MYH7 (HPA001239, Sigma-Aldrich, USA), Connexin 43 (3512S, Cell Signaling Technology, USA), SERCA2 (9580S, Cell Signaling Technology, USA), and beta-actin (ab8226, Abcam, UK), followed by secondary antibody staining (ab205718, Abcam, UK). Using chemiluminescence detection, the antibody-bound proteins were imaged. Quantification of protein expression levels were performed using Image J.

Basara G., Saeidi-Javash M., Ren X., Bahcecioglu G., Wyatt B.C., Anasori B., Zhang Y, & Zorlutuna P. (2020). Electrically conductive 3D printed Ti3C2Tx MXene-PEG composite constructs for cardiac tissue engineering. Acta biomaterialia, 139, 179-189.

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Western Blot Analysis of Cardiac Proteins

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Total protein was extracted from fresh tissues and cells based on the previous protocol (Song et al., 2021 (link)). Briefly, 50 μg protein samples were separated in 10% SDS-PAGE gel and transferred onto a nitrocellulose membrane. After 5% non-fat milk blocking, the blots were incubated with primary antibodies including BNP (1:200 dilution, sc-271185, Santa Cruz), β-MHC (1:500 dilution, HPA001239, Sigma), PI3K (1:2,000 dilution, A12484, Abclonal), LC3-II (1:2,000 dilution, A11534, Abclonal), Akt (1:2,000 dilution, A16343, Abclonal), and β-actin (1:5,000 dilution, KC5A08, Kangcheng) at 4°C overnight. Imaging System (LI-COR Biosciences) and ImageJ were used to perform a statistical analysis.

Song C., Zhang J., Liu Y., Hu Y., Feng C., Shi P., Zhang Y., Wang L., Xie Y., Zhang M., Zhao X., Cao Y., Li C, & Sun H. (2022). Characterization and Validation of ceRNA-Mediated Pathway–Pathway Crosstalk Networks Across Eight Major Cardiovascular Diseases. Frontiers in Cell and Developmental Biology, 10, 762129.

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