Mouse gm csf 415 ml

Foetal bovine serum (P30-5500; FBS) was purchased from Pan-Biotech (Aidenbach, Germany). Platelet activating factor (60900; PAF) was purchased from Cayman Chemicals (Ann Arbor, Michigan, United States), hydrogen peroxide (BA5193111; H₂O₂) from POCH (Gliwice, Poland), mouse GM-CSF (415-ML) from R&D Systems (Minneapolis, MN, USA), HEPES (BE17-737E) from Lonza (Basel, Switzerland) and mouse C5a (HC1101) from Hycult Biotech (Uden, The Netherlands). Roswell Park Memorial Institute (11835-063; RPMI) 1640 medium, SYTOX Orange (S11368), ionomycin (I4222) and E. coli BioParticles (E2861; K-12 strain) were purchased from Thermo Fisher Scientific (Waltham, Massachussets, United States). Calcium ionophore A23187 (C7522; CI), lipopolysaccharides (L2755; LPS) isolated from E. coli, Cytochalasin B (C6762) from Drechslera dematioidea, dihydrorhodamine 123 (D1054), DNAse (DN25) and phorbol 12-myristate 13-acetate (P1585, PMA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents, if not stated otherwise, were purchased from Becton Dickinson (BD; Franklin Lakes, NJ, USA).

Diphtheria toxin (DT) from Corynebacterium diphtheriae (Sigma-Aldrich, St Louis, MA, USA) was aliquoted and stored at -80 °C. The CM-H₂-DCFDA fluorescent probe (Molecular Probes, Eugene, OR, USA) was resuspended in dimethyl sulfoxide (DMSO) (Sigma-Aldrich) to a 1 mM concentration, aliquoted and stored at -20 °C. InVivoMAb anti-mouse IL-10 antibody (JES5-2A5 clone) and InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase (both from BioXcell, Lebanon, NH, USA) were stored at 4 °C. Mouse GM-CSF (415-ML) was from R&D Systems (Minneapolis, MN, USA), mouse C5a (HC1101) were purchased from Hycult Biotech (Uden, The Netherlands), and cytochalasin B (C6762) from Drechslera dematioidea was purchased from Sigma-Aldrich.