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Agilent bioanalyzer nano drop spectrometer

Manufactured by Agilent Technologies
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The Agilent Bioanalyzer and NanoDrop Spectrometer are analytical instruments designed for the assessment of biomolecules. The Bioanalyzer provides automated electrophoretic separation and detection of DNA, RNA, and proteins. The NanoDrop Spectrometer measures the concentration and purity of nucleic acid and protein samples using UV-Vis spectroscopy.

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Lab products found in correlation

Cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Purelink rna isolation kit, by Thermo Fisher Scientific (2 mentions) Dneasy blood and tissue isolation kit, by Qiagen (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions)

2 protocols using agilent bioanalyzer nano drop spectrometer

1

RNA and DNA Isolation for Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was prepared using the pure link RNA Isolation Kit (Invitrogen) or Trizol (Invitrogen). DNA was isolated using the DNeasy Blood and tissue isolation kit according to manufacturer’s protocol (Qiagen). The quality and concentration of RNA and DNA was determined by Agilent Bioanalyzer/Nano Drop Spectrometer. Isolated and purified total RNA was reverse transcribed by cDNA synthesis kit (Invitrogen). Taqman and CT kit was used for real time expression from the sorted cells. Real time PCR primers, DNMT1 (Mm01151063_m1), and HPRT (Mm01545399_m1), were designed either from applied biosystem site or Harward primer bank site (β-casein, ID1, ID2 and 18s).
Pathania R., Ramachandran S., Elangovan S., Padia R., Yang P., Cinghu S., Veeranan-Karmegam R., Arjunan P., Gnana-Prakasam J.P., Fulzele S., Pei L., Chang C.S., Choi H., Shi H., Manicassamy S., Prasad P.D., Sharma S., Ganapathy V., Jothi R, & Thangaraju M. (2015). DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis. Nature communications, 6, 6910.
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2

RNA and DNA Isolation for Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was prepared using the pure link RNA Isolation Kit (Invitrogen) or Trizol (Invitrogen). DNA was isolated using the DNeasy Blood and tissue isolation kit according to manufacturer’s protocol (Qiagen). The quality and concentration of RNA and DNA was determined by Agilent Bioanalyzer/Nano Drop Spectrometer. Isolated and purified total RNA was reverse transcribed by cDNA synthesis kit (Invitrogen). Taqman and CT kit was used for real time expression from the sorted cells. Real time PCR primers, DNMT1 (Mm01151063_m1), and HPRT (Mm01545399_m1), were designed either from applied biosystem site or Harward primer bank site (β-casein, ID1, ID2 and 18s).
Pathania R., Ramachandran S., Elangovan S., Padia R., Yang P., Cinghu S., Veeranan-Karmegam R., Arjunan P., Gnana-Prakasam J.P., Fulzele S., Pei L., Chang C.S., Choi H., Shi H., Manicassamy S., Prasad P.D., Sharma S., Ganapathy V., Jothi R, & Thangaraju M. (2015). DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis. Nature communications, 6, 6910.
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