Ginkgetin

Human AKT1, EGFR, and VEGF165 proteins were obtained from ACROBiosystems (Beijing, China), and human TP53 protein was purchased from Active Motif (Shanghai, China). Berberine (CAS: 2086-83-1), quercetin (CAS: 117-39-5), and daucosterol (CAS: 474-58-8) were purchased from Solarbio (Beijing, China), and ginkgetin (CAS: 481-46-9) and isoginkgetin (CAS: 548-19-6) were purchased from MedChemExpress (Shanghai, China). KH₂PO₄, NaCl, Na₂HPO₄, and KCl were obtained from Sinopharm Chemical Reagent (Shanghai, China), and dimethyl sulfoxide (DMSO) was purchased from Sangon Biotech (Shanghai, China).
The Series S Sensor Chip CM5 and Amine Coupling kit were obtained from GE Healthcare (Little Chalfont, UK). The prepared buffers were the running buffer (pH 7.4): 1 × PBS (2 mM KH₂PO₄, 10 mM Na₂HPO₄, 137 mM NaCl, and 2.7 mM KCl) with 0.05% Tween 20 and 5% DMSO and immobilization buffer (pH 4.0): 10 mM sodium acetate.

Ginkgetin (CAS No.: 481-46-9) and 2-hydroxy-benzylidene (2-HBA; CAS No.: 131359-24-5) were purchased from MedChemExpress (Shanghai, China). MTT was obtained from Sigma-Aldrich (St. Louis, MO, USA). Radio immunoprecipitation assay (RIPA) lysis buffer (P0013K) and BCA Protein Assay Kit (P0011) were obtained Beyotime Institute of Biotechnology (Shanghai, China). The anti-Bcl-2 (sc-7382) and anti-Bax (sc-7480) antibodies were purchased from Santa Cruz Biotechnology (CA, USA). The anti-Nox 2 (19013-1-AP), anti-Nox 4 (14347-1-AP), anti-GAPDH (10494-1-AP), and anti-cytochrome C (10993-1-AP) antibodies were obtained from Proteintech Group (Chicago, IL, USA). The anti-NF-κB (ab194726), anti-pro-caspase-9 (ab184786), anti-caspase-9 (ab52298), anti-caspase-3 (ab13847), and anti-pro-caspase-3 (ab184787) antibodies were purchased from Abcam (Cambridge, UK). HRP-conjugated Affinipure Goat Anti-Mouse IgG (SA00001-1) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2) were obtained from Proteintech Group (Chicago, IL, USA).