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2 protocols using cerium acetate hydrate

1

Cerium Compounds for α-Glucosidase Inhibition

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For the synthesis, cerium(iii) nitrate hexahydrate (Ce(NO3)3·6H2O, 99%), cerium(iii) chloride heptahydrate (CeCl3·7H2O, 99%), and cerium acetate hydrate (Ce(CH3COO)3, 99%) and cerium(iv) oxide were all obtained from Sigma-Aldrich. Diethylene glycol (C4H10O3, 99%) was obtained from Alfa Aesar. Throughout the reaction, double distilled water (purified using Aquatron, England) was used. For α-glucosidase inhibition activity of CeO2, α-glucosidase (≥50 units per mg protein), (PNPG, C12H15NO8, 99%), acarbose (C25H43NO18, ≥95%), sodium carbonate (Na2CO3, ≥99.5%), sodium phosphate dibasic heptahydrate (Na2HPO4·7H2O) and sodium phosphate monobasic monohydrate (NaHPO4·H2O, ≥99%) were obtained from Sigma-Aldrich. The cytotoxicity tests were carried out using MTT assay (3,4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide and RAW 264.7 cell lines (mouse macrophage) obtained from American Type of Culture Collection (ATCC, Rockville, MD, USA); penicillin/streptomycin, and fetal bovine serum (FBS) from GIBCO™, Invitrogen Corporation, USA; and dimethyl sulfoxide were obtained from Sigma-Aldrich.
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2

Synthesis and Evaluation of CeO2 Nanoparticles

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For the synthesis
of CeO2 NPs, cerium(III) nitrate hexahydrate (Ce(NO3)3·6H2O, 99%), cerium(III) chloride
heptahydrate (CeCl3·7H2O, 99%), and cerium
acetate hydrate (Ce(CH3COO)3·H2O, 99%) were obtained from Sigma-Aldrich. Commercial CeO2 (C-com) used for comparison was obtained from Sigma-Aldrich. Diethylene
glycol (C4H10O3, 99%) as a solvent
in the synthesis was purchased from Alfa-Aesar. Double-distilled water
that has been purified (Aquatron, England) was used throughout the
experiment. α-Glucosidase (≥50 units/mg protein), 4-nitrophenyl
α-d-glucopyranose (PNPG, C12H15NO8, 99%), acarbose (C25H43NO18, ≥95%), sodium carbonate (Na2CO3, ≥99.5%), sodium phosphate monobasic monohydrate (NaHPO4·H2O, ≥99%), and sodium phosphate dibasic
heptahydrate (Na2HPO4·7H2O)
were all obtained from Sigma-Aldrich for the α-Glucosidase inhibitory
study. RAW 264.7 cell lines (mouse macrophage) were purchased from
the American Type Culture Collection (ATCC, Rockville, MD) for the
cytotoxicity investigation. Sigma-Aldrich supplied the cytotoxicity
assays penicillin, streptomycin, fetal bovine serum (FBS), dimethyl
sulfoxide, and MTT (3,4,5-dimethylthiazol-2-yl)-2–5-diphenyl
tetrazolium bromide.
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