All reagents including sphingosine-1-phosphate (S1P) were purchased from Sigma-Aldrich unless otherwise specified. Imatinib was obtained commercially from LC Laboratories and reconstituted in DI water to yield a 20 mM stock. Dulbecco’s phosphate buffered saline (PBS) and trypsin were purchased from Life Technologies, while biological grade glutaraldehyde and paraformaldehyde used for cell fixation were obtained from Electron Microscopy Science. The following reagents and antibodies were acquired for immunofluorescence: mouse monoclonal anti-cortactin (p80/85) antibody clone 4F11 (EMD Millipore), mouse anti-paxillin antibody clone 349 (BD Biosciences), mouse anti-VE-cadherin antibody (Santa Cruz), Alex Fluor 488 goat anti-mouse IgG secondary antibody (Life Technologies), and Rhodamine-conjugated phalloidin (Molecular Probes).
Biological grade glutaraldehyde
Manufactured by Electron Microscopy Sciences
Biological grade glutaraldehyde is a chemical fixative used to preserve biological samples for analysis under an electron microscope. It is a colorless liquid that functions by cross-linking proteins within cells, which helps maintain the cellular structure and ultrastructure. This product is suitable for use in electron microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (2 mentions)
Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (2 mentions)
Rhodamine conjugated phalloidin, by Thermo Fisher Scientific (2 mentions)
Mouse monoclonal anti cortactin p80 85 antibody clone 4f11, by Merck Group (2 mentions)
Mouse anti paxillin antibody clone 349, by BD (2 mentions)
Mouse anti ve cadherin antibody, by Santa Cruz Biotechnology (2 mentions)
Alex fluor 488 goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (2 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)
Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions)
Imatinib, by LC Laboratories (1 mentions)
Hrp conjugated β actin antibody, by Proteintech (1 mentions)
Xfect rna transfection reagent, by Takara Bio (1 mentions)
2 protocols using biological grade glutaraldehyde
1
Cytoskeletal Dynamics in Endothelial Cells
2
Endothelial Cell Cytoskeleton Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Reagents were purchased from Sigma-Aldrich (e.g., S1P, thrombin) except as noted below. Dulbecco’s phosphate buffered saline (PBS) and trypsin were obtained from Life Technologies, while biological grade glutaraldehyde and paraformaldehyde were obtained from Electron Microscopy Science. The following reagents and antibodies were acquired for immunofluorescence: mouse monoclonal anti-cortactin (p80/85) antibody clone 4F11 (EMD Millipore), mouse anti-paxillin antibody clone 349 (BD Biosciences), mouse anti-VE-cadherin antibody (Santa Cruz), Alex Fluor 488 goat anti-mouse IgG secondary antibody (Life Technologies), HRP-conjugated β-actin antibody (Proteintech Group Inc), and Rhodamine-conjugated phalloidin (Molecular Probes). Scrambled and c-Abl specific siRNA was obtained from Santa Cruz Biotechnology, and Xfect RNA transfection reagent was obtained from Takara Clontech.
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