Trichloroacetic acid tca

NA, ACh, L-NAME, and Indomethacin were purchased from Sigma-Aldrich. Ketamine and midazolam were obtained from Pfizer. Streptozocin was purchased from Sigma-Aldrich. The Krebs-Henseleit solution (composition: 113 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO₄ × 7H₂O, 22 mM NaHCO3, 1.2 mM KH₂PO₄, 11 mM glucose, 2.5 mM CaCl₂ × 2H₂O, and 0.026 mM ethylenediaminetetraacetic acid (EDTA) (pH 7.4)) was prepared from EDTA and purchased from Sigma-Aldrich. CaCl₂ × 2H₂O and NaHCO₃ were purchased from Merck KGaA, Darmstadt, Germany, with the rest of the chemicals purchased from Kemika, Zagreb, Croatia. MS-PPOH was a gift from John R. Falck, Southwestern Medical Center, Dallas, TX, USA. The chemicals used to determine the oxidative stress were thiobarbituric acid (TBA; Sigma-Aldrich, DE, USA), trichloroacetic acid (TCA; Panreac, Europe), and 1,1,3,3-tetramethoxypropane (TMP; Sigma-Aldrich).

All chemicals were of analytical grade. Trichloroacetic acid (TCA) was obtained from PanReac AppliChem (Darmstadt, Germany). Chloroform and methanol were obtained from Nevareactiv (St. Petersburg, Russia). Sodium hydroxide (NaOH) and potassium chloride (KCl) were purchased from Uralhiminvest (Ekaterinburg, Russia).