β actin nb600 501

After imaging, brain tissues were lysed in ice-cold radioimmunoprecipitation assay buffer and total protein lysates were fractionated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes. The membranes were probed with antibodies against iba-1 (GTX100042) from GeneTex Biotechnology (Irvine, CA) at 1/1000 dilution, and TSPO (NB100-41398) from Novus Biologicals (Centennial, CO, USA) at 1/10,000 dilution and β-actin (NB600-501) from Novus Biologicals (Centennial, CO, USA) at 1/1000 dilution. After washing three times for 10 min in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h, washed with TBST and the signals were visualized and quantified using the GeneGnome Chemiluminescence Imaging System (Syngene, Frederick, MD, USA).