Gel documentation imaging system

Manufactured by Vilber

Sourced in France

The Gel documentation imaging system is a laboratory instrument designed for capturing and analyzing images of electrophoresis gels, such as those used in DNA, RNA, or protein analysis. The system includes a specialized camera, lighting, and software for capturing, processing, and storing the images.

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Lab products found in correlation

Spss 24, by IBM (1 mentions) Caspase 8, by MyBioSource (1 mentions)

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Gel documentation system (23 citations) Gel imaging system (9 citations)

2 protocols using gel documentation imaging system

Rapid Phylotyping of E. coli Isolates

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In this study, the E. coli strains were phylotyped by Clermont et al. (2013) scheme using a quadriplex PCR [10 (link)]. The sequences arpA (400 bp), chuA (288 bp), yjaA (211 bp) and TspE4.C2 (152 bp) were targeted to determine phylotypes including A, B1, B2, C, D, E, F and cryptic clade I. The strain EcoR62 was used as positive control in PCR examinations. Samples were subjected to a 35 PCR cycles including 10 s denaturation at 94 °C, 25 s annealing at 59 °C, and 5 s elongation at 72 °C. The PCR products were electrophoresed on 1.3% agarose gel for 60 min at 80 V. The electrophoresed gel was analyzed by gel documentation imaging system (vilber lourmat, France).
All data related to the presence or absence of phylogenetic groups and antibiotic resistance in each isolate were entered into Excel (Microsoft 2016) and SPSS (SPSS 24; IBM) programs to calculate the prevalence percentage in descriptive statistics.

Afsharikhah S., Ghanbarpour R., Mohseni P., Adib N., Bagheri M, & Jajarmi M. (2023). High prevalence of β-lactam and fluoroquinolone resistance in various phylotypes of Escherichia coli isolates from urinary tract infections in Jiroft city, Iran. BMC Microbiology, 23, 114.

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Protein Expression Analysis via Western Blot

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The following antibodies were used to test the protein levels of BIP (Cat# MBS857422, Mybiosource, USA), CHOP (Cat# MBS126028, Mybiosource, USA), Caspase-8 (Cat# MBS8808615, Mybiosource, USA), and LCIII (Cat# MBS2520564, Mybiosource, USA). Nanodrop was used to assay total protein levels (NanoDrop™ Lite Spectrophotometer, ThermoFisher Scienti c). Fifty micrograms of protein was added to SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). After electrophoresis, the proteins were transferred to a PVDF (polyvinylidene di uoride) membrane, incubated with primary antibodies, and targeted with secondary antibodies. Images of the membranes were captured using a gel documentation imaging system (Vilber, France). Using ImageJ software for densitometry, the resulting bands were quanti ed for their corresponding protein.

Ghorab D., Abu-El-Rub E.M., Gharaibeh M.H., Khasawneh R.R., Almazari R.A., Al-Emam A, & Helaly A.M. (2023). The toxic profile of tramadol combined with nicotine on the liver and testicles: evidence from endoplasmic reticulum stress. Molecular biology reports, 50(12).

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