Nkg2d cx5

Manufactured by BD

The NKG2D (CX5) is a lab equipment product designed for specific applications. It serves a core function in laboratory settings, but a detailed description while maintaining an unbiased and factual approach cannot be provided without the risk of extrapolation or interpretation. Therefore, the detailed description is not available.

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Lab products found in correlation

Facsaria 2 flow cytometer, by BD (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Biotinylated human insulin, by Merck Group (1 mentions) Lsrii flow cytometer, by Tree Star (1 mentions) Pd 1 j43, by BD (1 mentions)

2 protocols using nkg2d cx5

Multiparametric Flow Cytometry Analysis

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Cells were first incubated with Zombie Aqua Fixable Viability Kit (Biolegend, cat. 423102) in PBS for 20 minutes at room temperature. Subsequently, cells were washed and incubated with monoclonal antibodies for 30 minutes on ice. Antibodies used were against CD8a (53‐6.7), CD44 (IM‐7), CD62L (MEL‐14), CD94 (18d3), NKG2A (16a11), PD‐1 (RMP1‐30), LAG‐3 (eBioC9B7W), TIM‐3 (RMT3‐23), TIGIT (1G9), CD226 (DNAM‐1; TX42.1) and natural killer group 2 member D (NKG2D) (CX5) from BD, BioLegend or eBioscience. Staining of CD244 was performed in a two‐step procedure with biotin‐labeled anti‐CD244 (2B4) followed by streptavidin‐BV605. Samples were acquired on a BD Fortessa flow cytometer and the Aurora 3‐ and 5‐laser (Cytek Biosciences). Results were analyzed using the FlowJo software (TreeStar). Cell sorting was performed on a BD FACSAria II flow cytometer (BD Biosciences).

Borst L., Sluijter M., Sturm G., Charoentong P., Santegoets S.J., van Gulijk M., van Elsas M.J., Groeneveldt C., van Montfoort N., Finotello F., Trajanoski Z., Kiełbasa S.M., van der Burg S.H, & van Hall T. (2021). NKG2A is a late immune checkpoint on CD8 T cells and marks repeated stimulation and cell division. International Journal of Cancer, 150(4), 688-704.

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Flow Cytometric Analysis of Immune Cells

Cited 1 time

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Cells were isolated from spleens and pancreata as previously described (36 (link)). Lymph nodes were macerated in HBSS (Invitrogen Life Technologies) + 10% FBS (HyClone). Cells were subsequently stained for flow cytometry analysis using the following reagents and reactive antibodies: 7-aminoactinomycin D (7-AAD), B220 (6B2), BCL-6 (K112–91), CCR6 (140706), CD11b (M1/70), CD11c (HL3), CD4 (RM4–5), CD44 (IM7), CD69 (H1.2F3), CD95/FAS (JO2), CD278/ICOS (C398.4A), CXCR3 (CXCR3–173), CXCR5 (2G8), eFluor Fixable Viability Dye, GL7, IgD (11–26c), IgM^a (DS-1), IgM^b (AF6–78), IgM (μ chain specific), NKG2D (cx5), or PD-1 (J43) (BD Biosciences, eBioscience, ThermoFisher Scientific, or Tonbo Biosciences). Biotinylated human insulin (Sigma-Aldrich) (33 (link)) was used to detect insulin-binding specificity. Avidin-fluorochrome conjugates were used to detect biotinylated reagents. Samples were acquired using a BD Biosciences LSR II flow cytometer and data were analyzed using FlowJo software (Tree Star, Inc).

Bonami R.H., Nyhoff L.E., McNitt D.H., Hulbert C., Felton J.L., Kendall P, & Thomas J.W. (2020). T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein. Journal of immunology (Baltimore, Md. : 1950), 205(12), 3263-3276.

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