All surgical procedures were performed under general chloral hydrate anesthesia (SIGMA; 360 mg/kg, i.p.), complemented by infiltration of the postauricular region with local anesthetic (Xylocain; 1% AstraZeneca, Wilmington, DE, USA) and systemic analgesia (5 mg/kg Carprofen, s.c., CP-Pharma, Burgdorf, Germany). Rats were placed in a lateral recumbent position, the skin incised for an approximate length of 3 cm, the muscles pulled caudally and the bulla exposed. A slow speed 0.5 mm diameter diamond burr was used to drill a small hole into the bulla to display the round window, the tympanic cavity, and the ossicles. For the administration of neomycin via the round window, 5 mg neomycin was dissolved in 1 ml of PBS (adjusted to pH 7.4). A lancet was used to perforate the round window and a 30-gauge needle and micro syringe (Hamilton) were used to inject 15 μl neomycin (Wefstaedt et al., 2006 (link)). The bullostomy was closed with dental cement and the skin was sutured. The same procedure was repeated for the second ear. Animals received systemic analgesia at the day of surgery and for two more days subsequently (2.5 mg/kg Carprofen s.c.).
Carprofen
Manufactured by CP-Pharma
Sourced in Germany
Carprofen is a non-steroidal anti-inflammatory drug (NSAID) primarily used as a veterinary medication. It is an analytical standard for pharmaceutical laboratories and research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using carprofen
1
Neomycin Administration via Round Window
2
Transverse Aortic Constriction Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LVPO was induced by TAC in 9‐week‐old mice.
22 (link) Mice were subcutaneously pretreated with 1 mg/kg buprenorphine (Bayer, Leverkusen, Germany) and 5 mg/kg carprofen (Cp‐Pharma, Burgdorf, Germany). Anesthesia was induced with 5% isoflurane, sustained with 2% isoflurane, and monitored by toe pinching. Eye care solution was applied to prevent corneal injury and mice were placed in the supine position on a heating pad (37 °C). Mice were orally intubated and mechanically ventilated using a rodent ventilator (Hugo Sachs Elektronik, March‐Hugstetten, Germany). Following horizontal skin incision at the level of the suprasternal notch, the transverse aortic arch was visualized through a median sternotomy. Next, the transverse aortic arch was ligated using a 6–0 silk suture that was tightened around a blunt 25‐G needle, which was placed between the innominate artery and the left common carotid artery. The needle was quickly removed, leaving a discrete region of stenosis. The chest and overlying skin were closed. Animals received metamizole (1A Pharma, Holzkirchen, Germany) in the drinking water (200 mg/kg) for 4 days total, starting the day before surgery. The sham procedure was performed identically, except no suture was placed around the aortic arch.
22 (link) Mice were subcutaneously pretreated with 1 mg/kg buprenorphine (Bayer, Leverkusen, Germany) and 5 mg/kg carprofen (Cp‐Pharma, Burgdorf, Germany). Anesthesia was induced with 5% isoflurane, sustained with 2% isoflurane, and monitored by toe pinching. Eye care solution was applied to prevent corneal injury and mice were placed in the supine position on a heating pad (37 °C). Mice were orally intubated and mechanically ventilated using a rodent ventilator (Hugo Sachs Elektronik, March‐Hugstetten, Germany). Following horizontal skin incision at the level of the suprasternal notch, the transverse aortic arch was visualized through a median sternotomy. Next, the transverse aortic arch was ligated using a 6–0 silk suture that was tightened around a blunt 25‐G needle, which was placed between the innominate artery and the left common carotid artery. The needle was quickly removed, leaving a discrete region of stenosis. The chest and overlying skin were closed. Animals received metamizole (1A Pharma, Holzkirchen, Germany) in the drinking water (200 mg/kg) for 4 days total, starting the day before surgery. The sham procedure was performed identically, except no suture was placed around the aortic arch.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!